Studies on the functions of the actirated lymphocytes after partial hepatectomy

我们观察到，在C3H小鼠脾细胞中，部分肝切除术（HEP）增强了自然杀伤细胞（NK）的活性和淋巴因子激活杀伤细胞（LAK）的体外诱导能力。从肝切除小鼠脾细胞诱导的LAK细胞（HEP-LAK）不仅对肿瘤细胞系具有细胞毒性，而且对再生肝实质细胞（肝母细胞）和凝集素诱导的淋巴母细胞具有细胞毒性。此外，我们还发现HEP也增加了脾细胞IL-2的产生。因此，我们研究了LAK细胞对肝母细胞的细胞毒性在再生肝脏体内诱导的可能性。正常小鼠的非实质肝细胞和脾细胞对肝母细胞的细胞毒性很小。HEP后每日给予IL-2可显著增强其活性。密度梯度离心完全去除实质细胞的大鼠非实质肝细胞对肝母细胞表现出高的细胞毒活性。大鼠脾切除术后非实质细胞活性减弱。在体内实验系统中检测HEP-LAK细胞的细胞抑制活性。皮内注射HEP-LAK细胞可抑制霍乱毒素刺激的表皮增生。静脉注射HEP-LAK细胞抑制再生肝脏对氚胸苷的摄取。综上所述，淋巴细胞能够调节包括表皮细胞和肝细胞在内的正常上皮细胞的增殖，并发挥自组织作用。

We observed that activity of natural killer (NK) cells and in vitro inducibility of lymphokine activated killer (LAK) cells were augmented by partial hepatectomy (HEP) in the spleen cells of C3H mice. LAK cells induced from spleen cells of hepatectomized mice (HEP-LAK) were cytotoxic not only against tumor cell lines but also against parenchymal cells of regenerating liver (hepatoblasts) and lectin induced lymphoblasts. Further more, it was found that IL-2 production of spleen cells was also augmented by HEP. Therefore, we examined the possibility that LAK cells cytotoxic against hepatoblasts are induced in vivo in the regenerating liver. Non-parenchymal cells of liver and spleen cells obtained from normal mice exhibited little cytotoxicity against hepatoblasts. The activities were remarkably augmented by daily administration of IL-2 after HEP. Non-parenchymal liver cells of rat in which parenchymal cells are completely eliminated by density gradient centrifugation exhibited high cytotoxic activity against hepatoblasts. When rats were previously subjected to splenectomy, the activity of non-parenchymal cells after HEP were weakened. Cytostatic activity of HEP-LAK cells was examined in vivo experimental systems. HEP-LAK cells injected intradermaly suppressed epidermal proliferation stimulated by cholera toxin. HEP-LAK cells injected intravenously suppressed Tritium thymidine uptake of the regenerating liver. From above results, it was suggested that lymphocytes are able to regulate proliferation of normal epithelial cells including epidermal cells and liver cells, and play a role of auto-organization.