Effects of periodontally related microorganisms on metabolism of human fibroblasts from gingivae with various forms of periodontal disease

The purpose of this study is to evaluate the metabolism of human gingival fibroblasts from normal and various forms of periosontal disease for architectural components of periodontally related microorganisms.Ten cell lines of the gingival fibroblast were prepared from donors with different3types of periodontal disease and donors with healthy disease and donors with healthy disease and donors were prepared from donors with different3 types of periodontal disease and donors with healthy disease and donors were prepared from donors with different3 types of periodontal disease and donors。The cell lines were proliferated and stored in-80°C.Sonication extracts，sodium dodecyl sulphate extracts，and lipopolysaccharide components from the cell envelopes of Bacteroides gingivalis#381，B.asaccharolyticus ATCC25260，Actinobacillus actinomycetemcomitans Y4and ATCC29523，Capnocytophaga ochracea S3，Fusobacterium nucleatum ATCC 25586.The architural components 29523，Capnocytophaga ochracea S3，A fibroblast from juvenile periodontitis and a cell line from rapidly progressive periodontitis were greatly deformed in configuration，inhibited in cell adherence，and reduced in DNA synthesis by the components of A.actinomycescomitans。These results suggest that gingival fibroblast have different response to bacterial extracts among disease types of donors.

The purpose of this study is to evaluate the metabolism of human gingival fibroblasts from normal and various forms of periosontal disease for architectural components of periodontally related microorganisms.Ten cell lines of the gingival fibroblast were prepared from donors with different 3 types of periodontal disease and donors with healthy disease and donors with healthy gingiva : adult periodontitis, 3 cell lines ; rapidly progressive periodontitis, 2 cell lines ; juvenile periodontitis, 1 cell line ; and normal, 2 cell lines. The cell lines were proliferated and stored in-80゜C.Sonication extracts, sodium dodecyl sulphate extracts, and lipopolysaccharide components from the cell envelopes of Bacteroides gingivalis #381, B. asaccharolyticus ATCC 25260, Actinobacillus actinomycetemcomitans Y4 and ATCC 29523, Capnocytophaga ochracea S3, Fusobacterium nucleatum ATCC 25586.The architectural components from a variety of periodontally related microorganisms affected dose-dependently on various metabolic functions of fibroblast : cell configuration, cell adherence, glycosaminoglycan synthesis, collagen synthesis, or DNA synthesis. A fibroblast from juvenile periodontitis and a cell line from rapidly progressive periodontitis were greatly deformed in configuration, inhibited in cell adherence, and reduced in DNA synthesis by the components of A. actinomycescomitans. These results suggest that gingival fibroblast have different response to bacterial extracts among disease types of donors.