Immuno Molecular Analysis of Suppressor T cell receptor

包括抑制T细胞、辅助T细胞和杀伤T细胞在内的多种淋巴细胞功能相关抗原（LFA）对它们的分化和活化起着重要的作用。本项目利用cDNA编码LFAs和与LFAs反应的单克隆抗体，对LFAs的功能进行了表征。除CD8外，克隆小鼠CD2 cDNA，制备单克隆抗体。在表征良好的小鼠实验系统中，CD2的作用与从人类T细胞实验中获得的概念进行了比较。与人类系统相比，在它们的分布和功能方面观察到几个重要的差异。这些发现被认为对理解LFAs在免疫调节中的作用非常有用。除了LFAs的研究外，杀伤淋巴细胞的最终效应分子也从分子水平进行了研究。我们克隆了穿孔素cDNA，并利用克隆的cDNA对穿孔素的作用进行了广泛的研究，并在小鼠、大鼠和人的杀伤系统中发现了许多新的发现。

Including suppressor, helper and killer T cell, many kinds of lymphocyte function associated antigen(LFA) has been know to play important role for their differentiation and activation. In this project, experiments were performed to characterize the function of LFAs by using cDNA coding LFAs and monoclonal antibodies reactive with LFAs. Addition to CD8, murine CD2 cDNA was cloned and the monoclonal antibody was made. In the well characterized mouse experimental system, the role of CD2 was examined in comparison with the notion obtained from human T cells experiments. Several important differences were observed in terms of their distribuation and functions comparing to human systems. These finding were thought to be extremely useful to understand the role of LFAs in immune regulation. Beside LFAs study, the final effector molelcules of killer lymphocytes has also been studied from molecular level. cDNA cording perforin was cloned and the role of perforin was extensively studies by using cloned cDNA and various new findingds were abserved in mouse, rat and human killer systems.