The role of Japositive denditc cells in organ graht rejection

虽然表皮朗格兰细胞（epiderwal Langeilans cells， LC）在体外的作用已被广泛研究，但其在体内的作用仍存在争议。我们建立了一个精确的条件来诱导表皮细胞（EC）对同种异体抗原的延迟型高衰老（DTH）反应。为了阐明LC在体内的作用，我们首先使用UVB照射来减少这种DTH反应。我们还研究了中波紫外线对体内细胞毒性T淋巴细胞活性（CTL）的诱导作用和对移植物排斥反应的影响。在体外用UVB照射胫骨后，获得EC。这些EC通过皮下注射（DC）途径注射，并与注射后的EC进行DTH反应的比较。观察到的DTH切片减少（从62%到99.1%）。在体外刺激后评估这些小鼠的CTL活性。UVB辐照EC致敏小鼠体内CTL活性明显增强。此外，经upb辐照的小鼠对随后未经uvb辐照的异体造血干细胞排斥的同种异体造血干细胞没有产生排斥反应，并加速了移植的速度。接下来，我们将balb/c造血干细胞移植到（hbxbacr /c）细胞上，在移植后150天取出这些移植的蛋白并制成EC，这些EC注射到正常的balb/c小鼠体内。用抗β - k血清消耗β - k和补充β - k来研究β - k的作用。对于反应，抗血清+补剂处理小鼠无明显减少。在CTL活性方面，抗ta^k +补剂组CTL活性明显降低。当使用uvb时，可以观察到TDH的减少和morwel CTL的活性。因此，虽然lc对于DTH和CTL的诱导都是必需的，但当使用uvb照射时，可能会有其他机制起作用。

Althcugh in vitro role of epiderwal Langeilans cells (LC) has been studied extensitiely in vivo rile of LC is still a matter of controversy. In a preions paper, we have estellishied a precise condition to induce delayed-type hypersenoiticrty (DTH) reaction to alloantigens using epidermal cells (EC). In order to clauty the role of LC in vivo, we first used UVB inadiation for the reduction of this DTH reaction. The UVB effect on the induction of cytotoxic T lymphocyte activity (CTL) in vivo and on shingraft nrejection was also mivestigated. Aterthe shin was iisradiated with UVB in vitro, EC were obtainecl. These EC were iinjected via subcutanevus (DC) route and DTH reaction was compared to that mentreated EC. Reduction of DTH seaction who obsewed (fron 62 to 99.1%). CTL activity in these mice was assessed aften in vitrs stimulation. CTL actiorty in miee sensitized with UVB inadiated EC was apprecially seduced. Furtheimore mice scmityod with upb irsadiated EC did not reject the followingon-uvb-inadiated ec rejected auhsegnent shin allograftin an accelewthe feshionNext we used balb/c shin transpleuted onto (cbhxbacr/c)fimice, these jrefted plrin was taken 150 days aftir trimsplantation and ec were made, these ec were injected sc into mormil balb/c mice. The role of lc was studied by depleting lc with anti ta^k serum and c rnplement treatinent. For dth reaction significent reduction was notcd in anti ta^k serum + conplement treated mice. for ctl activity, appaeciable reduction who noted in anti-ta^k + conplement treated group. when uvb wao used reduced tdh reuction and morwel ctl activity was noticed. thus althcugh lc was necessauy for both dth and ctl induction, other mechauism wuned be operatig when uvbv irrediation was used.