Studies of genetic transformation in cereal plants

用含有编码氨基糖苷磷酸转移酶II（APH（3 ′）II）和CaMV 35 S启动子的嵌合基因的质粒DNA处理原植物，再生水稻植株。从悬浮细胞中分离得到的水稻原生质体，在质粒DNA的存在下，通过电穿孔或PEG处理。电穿孔原生质体的种植效率在三周后约为0.5%。将对照中形成的G418抗性菌落作为分裂细胞，将大于1 mm的愈伤组织转移到再生培养基上。从抗性愈伤组织中获得了几株再生植株。当在含有50 μ g/ml硫酸卡那霉素的培养基中生长时，这些植物的叶没有漂白。在转化植物的叶提取物中检测到APH（3 ′）Ⅱ活性。对转基因植株及其后代的DNA分析表明，在受体植株中存在完整形式的外源DNA。目前正在努力使外源DNA在水稻植株中整合的方式和位点可视化。

Rice plants were regenerated from protoplants treated with plasmid DNA possessing a chimeric gene encoding aminoglycoside phoshotransferase II (APH(3')II) and CaMV35S promoter. Rice protoplants isolatied from suspension cells were treated by electroporation or PEG in the presence of plasmid DNA. The planting efficiency of electroporated protplasts was about 0.5% after three weeks. The dividing cells were selected G418-resistant colonies formed in the control.Calli larger than l mm were transferred to regeneration medium. Severl plants were regenerated from drug resistant calli. Leaves of these plants were not bleached when grown in the medium containing 50 ug/ml Kanamycin sulphate APH(3')II activity was detected in the leaf extracs of transformed plants. Analysis of DNA of the trasgenic plants and progenies indicated the presence of indicated the presence of intact forms of foreign DNA in recipient plants.Other exampl of DNA transformation resulted in the production of transgenic plants will be pressented. Efforts are being made to visualize the manner and the sites of foreign DNA integration in rice plants.