A Study on Mass Culture of Plankton Living in Extreme Environments and their Production of Useful Compounds.

本项目的目的是为生活在极端环境中的浮游植物的大量繁殖建立最佳条件，并寻找这些浮游植物产生的生物活性化合物和含氮萃取成分等有用物质。收集并大量培育了30余种浮游植物。通过抑菌活性试验和抑制受精卵发育试验筛选生物活性化合物。3 .从生活在岩石池中的鞭毛藻假性腺瘤中分离到一种新的抗真菌聚醚大环内酯类化合物goniodomin A，并根据光谱数据对其结构进行了鉴定。对耐盐绿藻杜氏藻的提取氮成分进行了分析。大约45%的成分是由多肽组成的，其中一些在酶分析中显示出抑制作用。

The aim of the present project is to establish optimum conditions for massculture of phytoplankton living in extreme environments and to search for such useful substances as biologically active compounds and nitrogenous extractive componets produced by those plankton.1. More than 30 species of phytoplankton were collected and mass cultivated.2. Screening of biologically active compounds was carried out by means of the antimicrobial activity test and the inhibition test of development of fertilized echinderm eggs.3. A novel antifungal polyether macrolide, goniodomin A, was isolated from the dinoflagellate Goniodoma pseudogoniaulax living in the rock pool and its structure was determined on the basis of spectral data.4. Extractive nitrogenous constituents in the halotolerant green alga Dunaliella bardawil were analyzed. Approximately 45% of the constituents was accounted for by peptides, some of which showed inhibitory effects in the enzyme assay.