Confocal microscope for the fluorescent imaging of cells

荧光标记不仅在化学生物分析和分子诊断中发挥着越来越重要的作用，而且在快速发展的活细胞分子成像研究领域也发挥着越来越重要的作用。KIT各参与和筹备工作小组的研究训练组和许多其他第三方资助的研究项目都以这一主题为重点。这些研究小组没有共聚焦显微镜，特别是博士研究人员和同事无法独立进行荧光细胞成像的细胞生物学实验。这种显微镜实验只能在高度专业化和先进的显微镜上合作进行。这在很大程度上限制了细胞实验，因为参与本提案的主要准备工作研究小组对新合成和荧光探针和偶联物的研究，给专业合作伙伴带来了负担。特别是基本的实验，如转染研究、细胞内生物聚合物的荧光标记或细胞中基于能量转移的结构的观察，可以由博士研究人员自己独立进行，当然，在负责新显微镜的人的指导下。为此目的，不需要高度专业化的显微镜，这些显微镜是为高级研究而建造的，可以在没有参与本提案的合作专业研究小组中使用。利用所提出的基本显微镜，博士研究人员和同事可以独立于专业合作伙伴进行基本的应用实验和优化时间。注意，不仅获得了跨学科的能力，而且还节省了个别研究人员和整个项目的时间。博士研究人员已经带来了正确的和跨学科的能力，通过研究项目“化学生物学”在KIT。

Fluorescent labelling plays not only an increasing role in chemical bioanalytics and molecular diagnostics but also in the fast growing research area of molecular imaging of living cells. The research training group and many other third-party funded research projects of the participating and preparative working research groups at KIT focus on this subject. There is no confocal microscope available for these research groups and in particular not to the doctoral researchers and coworkers to independently perform cell biological experiments for fluorescent cell imaging. Such microscopy experiments must be performed only in collaboration and on highly specialized and advanced microscopes. This limits the cellular experiments very much, because the investigations of the newly synthesized and fluorescent probes and conjugates from the mainly preparative working research groups, who are participating in this proposal, overburden the specialized collaboration partners. Expecially the basic experiments, such as transfection studies, fluorescent labelling of biopolymers inside cells or the observation of energy transfer-based architectures in cells could be performed independently by the doctoral researchers themselves, of course after instruction by the person who is responsible for the new microscope. For this purpose, highly specialized microscopes are not necessary, which are built for advanced studies and available in the collaborating specialized research groups that are not participating in this proposal. With the proposed basic microscope the doctoral researchers and coworkers are able to perform basic application experiments and optimizations time independently from the specialized collaboration partner. Note only Interdisciplinary competences were gained but also time savings by the individual researchers and the overall projects. The doctoral researchers bring already the right and interdisciplinary competences by the curriculum of the studying program "Chemical Biology" at KIT.