Experimental Infection and Characterization of Epidemic Non-A, Non-B Hepatitis Virus.

建立流行性非甲、非乙型肝炎动物模型。食蟹猴是一个很好的动物模型，因为如果我们静脉接种足够数量的病毒，几乎所有的猴子都会患上急性肝炎。成功地进行了串口传输实验。我们发现，从接种后10d到转氨酶高峰期，大量病毒分泌到胆汁中。早期病毒散在分布于胆汁中，下一阶段病毒颗粒自发聚集，可能是由于分泌了IgA。我们已经用免疫筛选的方法从lambda gt11基因文库中克隆了病毒RNA。用聚合酶链式反应方法确认潜伏期病毒血症。目前，我们正在用重组多肽研制抗体检测试剂盒。我们也在尝试推出重组疫苗。

An attempt was made to establish the animal model of epidemic non-A, non-B hepatitis. Cynomolgus macaca was a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatits. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory IgA. We already cloned viral RNA from lambda gt11 cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to debelop recombinant vaccine too.