Glycosphingolipids of various human ovarian tumors

1.多种卵巢肿瘤的糖鞘糖脂(GSLS)：在卵巢肿瘤、粘液性囊腺癌、浆液性囊腺癌和透明细胞癌中，粘液性囊腺癌具有独特的GSL组成。粘液性囊腺癌中90%以上的酸性GSL由硫代脂类组成，在正常卵巢中很少检测到，而在其他类型的卵巢肿瘤中含量低于40%。通过负离子快原子轰击质谱仪(FABMS)和气-液色谱(GLC)分析，证实粘液性囊腺癌的主要硫脂成分为含N-脑磷脂的I^3SO_3-GalCer，而不是含N-非羟基脂肪酰鞘氨醇的I^3SO_3-GalCer。抗人卵巢癌单抗MRG-1：用人卵巢癌细胞免疫小鼠建立的小鼠单抗MRG-1，优先与细胞的高尔基复合体和微粒体部分反应，而不与质膜反应，而抗原分布在正常人宫颈上皮细胞间基质中。TLC免疫染色鉴定该抗原为中性GSLS，具有A血型决定簇。用原子吸收光谱和核磁共振谱鉴定其结构为ceramide-Glc4-Gal3-GlcNAc4-Gal(2-Fuc)3-GalNAc3-Gal(2-Fuc)3-GalNAc，IIIA型GSLs。对卵巢癌患者血清半乳糖基转移酶相关肿瘤(GAT)进行了临床研究。不同组织学类型的卵巢癌GAT阳性率均较高，透明细胞癌为55.0%，子宫内膜样腺癌为66.7%。良性疾病患者，尤其是子宫内膜异位症患者，GAT阳性率明显低于其他同时明确标记物的阳性率。这些结果表明GAT在鉴别卵巢肿瘤良恶性方面有一定的应用价值。

1. Glycosphingolipids (GSLs) of Various Human Ovarian Tumors:Among the human ovarian tumors, mucinous cystadenocarcinoma, serous cystadenocarcinoma, and clear cell adenocarcinoma, the mucinous cystadenocarcinoma showed a unique GSL composition. More than 90% of the acidic GSL in the mucinous cystadenocarcinoma was comprised of sulfolipids, rarely detected in normal ovary and found in concentrations of less than 40% in the other types of ovarian tumors. By means of negative ion fast atom bombardment mass spectrometry (FABMS) and gas-liquid chromatography(GLC), the major sulfolipid in mucinous cystadenocarcinoma was confirmed as I^3SO_3-GalCer with N-cerebronyl phytosphingosine, rather than I^3SO_3-GalCer with N-nonhydroxy fatty acyl sphingosine, the major molecular species in the other ovarian cancers.2. a Monoclonal Antibody, MRG-1:A murine monoclonal antibody, MRG-1, established by immunizing mouse with human ovarian carcinoma-derived cells, reacted preferentially with the cells' Golgi complexes and microsome fractions, but not with the plasma membranes, whereas the antigen was distributed in the intercellular matrix of normal human cervical epithelium. The antigen was identified as neutral GSLs with blood group A-determinant by TLC-immunostaining. Using FABMS and NMR, the structure was found to be ceramide-Glc4-Gal3-GlcNAc4-Gal(2-Fuc)3-GalNAc3-Gal(2-Fuc)3-GalNAc, type IIIA GSLs.3. Galactosyltransferase Associated with Tumor (GAT):GAT was studied clinically in ovarian cancer patients. The GAT positive rate for different histologic types of ovarian carcinoma was relatively high for each type, e. g., 55.0% for clear cell carcinoma and 66.7% for endometrioid adenocarcinoma. In patients with benign diseases, in particular endometriosis, the GAT positive rate was lower than the positive rate with any other simultaneously definite markers. These results indicate the usefulness of GAT in distinguishing malignant from benign ovarian tumors.