Study on the physiological function of bombyxin

1.我们制备了多种抗人工合成的家蚕毒素-Ⅱ的鼠源单克隆抗体，并建立了家蚕毒素的时间分辨荧光免疫分析法（TR-FIA）。结论：1.该方法灵敏度高、简便、安全、省时，上级常规放射免疫分析法。采用TR-FIA技术，研究了家蚕幼虫-蛹-成虫发育过程中血淋巴中家蚕毒素滴度的变化。幼虫期的滴度较低，而蛹的早期和中期较高。仅在雄性中羽化后立即观察到滴度急剧上升。羽化后血淋巴中的脂质含量也有所增加，但只有雄虫的血淋巴中的脂质含量有所增加，提示家蚕毒素可能参与了脂代谢的调节.本文研究了家蚕毒素在家蚕幼虫体内的生理功能，结果表明：1）家蚕毒素具有降低海藻糖酶活性的作用; 2）家蚕毒素能提高中肠和体壁的海藻糖酶活性（包括肌肉）。3）阿托伐他汀对脂肪体中的海藻糖合酶活性没有影响。4）这些结果表明，家蚕毒素调节糖和脂代谢，在脂肪组织中起重要作用在蜕变的过程中。

1. We produced many kinds of mouse monoclonal antibodies against synthetic bombyxin-II and developed a time-resolved fluoroimmunoassay (TR-FIA) for bombyxin. This assay method is superior to the conventional radioimmunoassay in sensitivity, simplicity, safety and time-saving.2. Using TR-FIA,we examined the changes in bombyxin titer in hemolymph during larva-pupa-adult development of the silkmoth, Bombyx mori. The titer was low in the larval stage, while high in the early and middle pupal stages. A steep rise in the titer was observed immediately after eclosion only in males. Hemolymph lipid also increased after eclosion only in males, suggesting a possibility that bombyxin is involved in the regulation of lipid metabolism.3. We investigated the physiological function of bombyxin in Bombyx larvae and obtained the following results.1) Bombyxin has a hypotrehalosemic activity.2) Bombyxin increases the trehalase activity in the midgut and integument (including muscles).3) Bombyxin has no effect on trehalose synthase activity in the fat body.4) Bombyxin decreases glycogen content in the fat body.These results suggested that bombyxin regulates sugar and lipid metabolism and that bombyxin plays an important role (s) in the process of metamorphosis.