A flow cytometric DNA analysis ofthe reversible testicular damage in the rat and the effect of mecobalamin on the recovery of spermatogenesis

本研究评价了DNA流式细胞术在检测和评价盐酸阿霉素(ADR)诱导的精子发生可逆性损伤中的应用。雄性SD大鼠(S-D)皮下注射阿霉素0.1、0.2 5、0.5 mg/kg，每周3次，共3、5、7周。对照组在相同的三种方案下接受生理盐水治疗。通过对大鼠体重和睾丸重量变化的评估以及DNA流式细胞仪分析，结果表明，0.25 mg/kg的ADR连续5周处理的大鼠最适合作为可逆性睾丸损伤模型。雄性S-D大鼠皮下注射阿霉素0.25 mg/kg，3次/周，连续5周后，给予MBL0.01 mg/kg，0.1 mg/kg，1 mg/kg，每周6次。治疗后5周、10周、15周取睾丸，进行流式细胞术DNA分析。在15周时，0.1 mg/kg的MBL处理组较对照组(仅ADR)恢复显著。这些结果表明，该动物模型可用于快速、定量地评估化学制剂对精子发生的影响，并且MBL促进了实验性损伤的精子生成的恢复过程。

This study evaluated the use of DNA flow cytometric analysis to detect and quality reversible damage in spermatogenesis induced by doxorubicin hydrochloride(ADR). Male Sprague-Dawley(S-D) rats were treated subcutaneously with ADR at a dose of 0.1, 0.25 or 0.5 mg/kg, 3 times a week for either 3, 5 or 7 weeks. Controls were treated with either saline following the same three regimens. Evaluation of changes in body and testicular weights, as well as DNA flow cytometric analysis, revealed that rats treated with ADR at 0.25 mg/kg for 5 weeks were the most suitable as a reversible testicular damage rat model.Standing on the above established animal model, the effect mecobalamin (MBL) on the recovery process of spermatogenesis was investigated. Male S-D rats were subcutaneously injected with ADR 0.25 mg/kg, 3 times week, for 5 weeks, after which rats were orally adiministered with MBL 0.01 mg/kg, 0.1 mg/kg and 1mg/kg, 6 times a week. Five, ten and fifteen weeks after the treatment, testicles extracted and flow cytometyric DNA analysis was performed. In the group treated with 0.1 mg/kg of MBL, significantly greater recovery was revealed compared with the control group(only ADR) at 15 week.These results suggest that this animal model is useful for rapid and quantitative assessment of the effect the chemical agenct on spermatogenesis and that MBL promoted the recovery process of experimentally damaged spermatogegesis.