Outflow facility : conventional and unconventional flow

为了进一步了解房水流出的机制，我们研究了几种药物对房水流出设施、眼内肌肉组织收缩和牛眼小梁网细胞形态变化的影响。它由一套2毫升的神经尖吸管组成，水平地固定在一个木框里。本文研究了细胞骨架药物、作用药物和自主神经药物对灌注眼的流出设施和培养的小梁网细胞形态的影响。对分离的眼内肌组织进行等长收缩测量，发现牛睫状肌中M_3受体占优势。盐酸莫昔洛特是一种1-肾上腺素受体阻滞剂，以前被称为胸腺嘧啶核苷，其作用是通过去甲肾上腺素的竞争性拮抗来实现的。这种药物在不影响眼压或睫状肌控制的流出设施的情况下导致缩瞳，我们检查了其效果。此外，位于牛睫状肌神经末梢的肾上腺素能2受体的激活抑制了胆碱支配的肌肉的收缩。M3受体和突触前2受体在睫状肌中起重要作用。我们的激光小梁切除术的疗效与传统的激光小梁切除术进行了比较。虽然我们的激光小梁刺激术没有激光小梁成形术有效(60%)，但这项试验可能会导致另一种有用的治疗方式的发展。尽管进一步的讨论还需要更多的数据，但这些结果表明，流出设施的变化以及伴随的睫状肌反应和细胞形状凹陷可能彼此密切相关。

To further understand the aqueous outflow mechanism, we examined the action of a variety of several drugs on the outflow facility, contraction of intraocular muscle tissues and cell shape change of bovine trabecular meshwork.A device capable of perfusing 10 eyes in parallel was made. It consisted of a set of 2ml nerrow-tipped pipetts held horizontally in a wooden frame. Changes in the outflow facility of perfused eyes and in the shape of cells in cultured trabecular meshwork were studied after exposure to several types of drugs including cell cytoskeleton acting drugs and autonomic drugs. Contraction of the isolated intraocular muscle tissues were measured isometrically.Muscarinic M3 receptor was dominant in the bovine ciliary muscle. Moxisylyte hydrochloride, an 1 -adrenoceptor-blocking agent previously called thymoxamine works by competitive antagonism of norepinephrine. The drug causes miosis without affecting the intraocular pressure or the ciliary muscle-controlled facility of outflow, and we examined the effects. Furthermore, adrenergic 2 receptor activation located at the nerve terminals of bovine ciliary muscle inhibited the contraction of the cholinergically innervated muscle. M3 receptor and pre-synaptic 2 receptor play an important role in the ciliary muscle. The efficacy of our laser traabeculostimulation was compared with that of conventional laser trabeculectomy. Although our laser trabeculostimulation was less effective(60%) than lasertrabeculoplasty, the trial may lead to the development of another useful treatment modality.Although further discussion must await additionl data, these results suggest that the changes in outflow facility and the accompanying ciliary muscle response and cell shape cahnge may be closely related to each other.