High resolution electrospray ionization tandem mass spectrometer coupled to nanoflow chromatography for proteome research

我们在哥廷根大学的大学医学中心(UMG)申请了一台高分辨率的电喷雾电离串联质谱仪，并与纳米流色谱联用，用于蛋白质组研究。我们正在申请的仪器将被放置在UMG的科学核心设施蛋白质组学(DFG RIsources RI_00483)中，以便优先向UMG的研究小组提供这项蛋白质组研究中的关键技术。Core设施于2012年在UMG临床化学研究所成立，目前正在运行两台高分辨率质谱仪。我们计划将新仪器主要用于以下应用：1)通过数据独立采集(DIA)质谱仪对细胞培养、体液和组织样本中的蛋白质组进行无标记分析2)亲和富集后翻译后蛋白质修饰如磷酸化和泛素化的全局分析3)通过亲和纯化质谱学或邻近图谱方法分析蛋白质相互作用、生物分子复合体和细胞室4)使用双功能交联剂通过化学交联剂分析蛋白质/蛋白质直接接触部位

We apply for a high resolution electrospray ionization tandem mass spectrometer coupled to nanoflow chromatography for proteome research at the University Medical Center Göttingen (UMG), University of Göttingen. The instrument we are applying for will be placed in the UMG's Scientific Core Facility Proteomics (DFG RIsources RI_00483) to offer access to this key technology in proteome research preferentially to the UMG's research groups. The Core Facility was founded in the UMG Institute of Clinical Chemistry in 2012, and is currently operating two high resolution mass spectrometers. The mass spectrometer applied for here will replace one of these aging instruments.We plan to mainly use the new instrument for the following applications:1) label-free proteome profiling in samples from cell culture, body fluids and tissues by data-independent acquisition (DIA) mass spectrometry2) global analysis of posttranslational protein modifications such as phosphorylation and ubiquitinylation after affinity enrichment3) analysis of protein interactions biomolecular complexes and cell compartments by affinity purification mass spectrometry or by proximity profiling methods4) analysis of direct protein/protein contact sites by chemical rosslinking using bifunctional crosslinking reagents