Study on Bioelastic Materials for Artificial Blood Vessel Derived from Elastic Fiber Materials

1993年合成了X-聚（GVGVP）、基于重复五肽序列Gly-Val-Gly-Pro（GVGVP）和X-聚[a（GVGVP），B（GRGDSP）]（含有共价结合的细胞粘附和血小板聚集抑制序列Arg-Gly-Asp-Ser（GRDS））的γ辐照交联弹性体材料。在1994年，这些材料被测试其支持牛主动脉内皮细胞的粘附和生长的能力。在含白蛋白的培养基中进行3小时的粘附实验表明，含有1：0.017、1：0.025和1：0.05比例的（GVGVP）：（GRGDSP）的材料支持最大的细胞粘附，含有1：0.01比例的（GVGVP）：（GRGDSP）的材料表现出中等水平的粘附，并且含有1：0.015比例的（GVGVP）：（GRGDSP）的材料支持最大的细胞粘附。0.002比率的（GVGVP）：（GRGDSP）和单独的（GVGVP）对于细胞附着是非常差的支持。在相同的培养基中进行24小时的细胞生长实验，结果表明：（GVGVP）：（GRGDSP）分别为1：0.017、1：0.025和1：0.05的材料对内皮细胞有三种群体倍增作用，而单独的（GVGVP）材料对内皮细胞生长的支持作用较差。

The X-poly (GVGVP), THE gamma-irradiation cross-linded elastomeric materials based on the repeating pentapeptide sequence Gly-Val-Gly-Pro (GVGVP) and X-Poly [a (GVGVP), b (GRGDSP)] containing the covalently incorporated cell-adhesion and platelet-aggregation-inhibition sequence Arg-Gly-Asp-Ser (GRDS) were synthesized in 1993. In 1994 these materials were tested for their ability to support the adhesion and growth of bovine aortic endothelial cells. Adhesion experiments carried out for 3h in albumin-containing media showed that materials containing, 1 : 0.017,1 : 0.025, and 1 : 0.05 ratios of (GVGVP) : (GRGDSP) supported maximal cell attachment, that the material containing 1 : 0.01 ratio of (GVGVP) : (GRGDSP) exhibited as intermediate level of attachment and that the material composed of 1 : 0.002 ratio of (GVGVP) : (GRGDSP) and (GVGVP) alone were very poor supports for cell attachment. Cell growth experiments cerried out for 24h insame media showed that materials containing 1 : 0.017,1 : 0.025, and 1 : 0.05 ratios of (GVGVP) : (GRGDSP) supported three population doublings of endotherials cell and material composed of (GVGVP) alone was poor support for cell growth.