Pilot Production of Artificial Membrane Vaccine

研究了人工膜疫苗的中试生产。首先，我们研究了两亲性MDP衍生物与磷脂酰胆碱组成的混合囊泡的形成和性质。MDP （muramyl二肽）是肽聚糖中最小的单位，具有细胞免疫和佐剂活性。采用去污剂法制备混合囊泡。含2条烃链的MDP衍生物含量小于40%的混合囊泡为单层囊泡，直径为200 nm。寡层小泡或纤维状结构占60%以上。含有含烃链的MDP衍生物的囊泡大小随着MDP衍生物含量的增加而减小。接下来我们研究了流感抗原蛋白在囊泡膜上的重组。以胆酸钠为表面活性剂的洗洁剂脱除法制备抗原蛋白囊泡时，重组效率最高。

A pilot production of artificial membrane vaccine was studied. First, we started a study on formation and property of mixed vesicles constituted of amphiphilic MDP derivatives and phosphatidylcholine. MDP (muramyl dipeptide) is the smallest unit of peptide glycan which possessed cell-mediated immunity and adjuvant activity. Mixed vesicles were prepared by detergent removal method. Mixed vesicles containing less than 40% of MDP derivatives with 2 hydrocarbon chains were unilamellar with 200 nm of diameter. Over 60% oligolamellar vesicles or fibriform structure apperared. The size of the vesicles containing a MDP derivative with a hydrocarbon chain was decreased with increasing MDP derivative content. Next we studied on reconstitution of influenza antigen protein on the vesicle membrane. The efficiency of the reconstitution of the antigen protein was the best when the vesicles were prepared by detergent removal method with sodium cholate as a surfactant.