SIGNIFICATION OF VOLATILE COMPOUNDS DETECTED IN BODY TISSUES

1)煤油成分的测定毛细管气相色谱/质谱仪(GC/MS)与正戊烷溶剂萃取法相结合，建立了一种灵敏、快速的分析煤油成分的方法，可在接触煤油蒸气的人体组织中清晰地检测到碳数大于9的脂肪烃和甲苯、二甲苯、三甲苯等芳香族化合物。结果表明，该方法可用于人体组织中燃料成分的检测。2)人体组织中煤油成分水平与吸入煤油蒸气浓度的关系。大鼠暴露于普通玻璃瓶和管子制成的煤油蒸气中5或15分钟。选择组织中的异丙苯、异丙苯、均三甲苯和1，2，3-三甲苯等三甲苯作为指示剂，推测煤油蒸汽暴露的浓度。四种化合物的血液浓度随着吸入煤油蒸汽的时间的增加而成比例地增加，在其他身体组织中也观察到了几乎相同的趋势。在煤油作用5min和15min时，肺中的三甲苯浓度与血液中的浓度几乎相同，因此，当血液因分解而无法采集时，肺是最具替代性的样品。

1) Determination of kerosene componentsA sensitive and rapid method to analyze kerosene components was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.The aliphatic hydrocarbons with carbon number over 9 and aromatics such as toluene, xylene and trimethylbenzenes were clearly detected in body tissues exposed to kerosene vapor. The lower limit of detection was ca. 10 pg in each of trimetylbenzenes.These result suggest that this method is useful to detect fuel components in body tissues.2) Relationship between the levels of kerosene components in body tissues and the concentration of kerosene vapor inhaledRats were exposed to kerosene vapor (1,000 ppm) in a chamber made by using common glass bottles and tubes for 5 or 15 min. Trimethylbenzenes including cumen, puseudocumene, mesitylene and 1,2,3-trimethylbenzene in tissues were selected as indicators to presume the concentration of kerosene vapor exposed. Blood levels of the four compounds increased in proportion to the inhalation time of kerosene vapor, and almost the same tendency was observed in the other body tissues. The concentrations of trimethylbenzenes in the lung were nearly the same as those in the blood either for 5 or 15 min-exposure to kerosene, so that the lung would be the most substitutive sample when blood could not be collected due to decomposition.