A neuroendocrinological study for rats exposed to emotional stress as a model of anorexia nervosa.

通讯箱系统（1.5mA/秒，2次/分，1小时/次，2次/天，连续7天）长期暴露于情绪应激条件下，Wister品系大鼠的总摄食量和体重增加明显减少。这种压力还会降低血糖，增加血浆ACTH和皮质酮水平。长期暴露于情绪应激的大鼠在熟悉环境中表现出运动活动增加，在新环境中表现出探索行为减少。慢性情绪应激大鼠下丘脑免疫反应性促肾上腺皮质激素释放因子（CRF）含量显著升高。原位杂交观察到，5d的情绪应激只诱导大鼠室旁核旁细胞神经元中CRF m RNA的表达。通过脑内微透析检查，暴露于情绪应激可增加室旁核去甲肾上腺素的释放。实验第1天5min情绪应激诱导的去甲肾上腺素升高在第7天重复相同应激时没有变化，说明去甲肾上腺素神经元未发生脱敏。这些变化是由长期暴露于情绪压力引起的，而不是由电击引起的。在我们的研究中使用的情绪应激在大鼠中引起的结果与神经性厌食症患者相似，但性腺功能减退症伴有室旁核CRF和去甲肾上腺素释放的激活。

The chronic exposure to emotional stress using by a communications box system (1.5mA/sec, 2times/min, 1hr/time, 2times/day for 7day) significantly reduced total amount of food intake as well as body weight gain in Wister strain's rats. That stress also decreased blood sugar and increased plasma levels of ACTH and corticosterone. Rats chronically exposed to emotional stress showed increased locomotor activity in a familiar environment and decreased exploratory behaivior in a novel environment. The content of immunoreactive corticotropin-releasing factor (CRF) significantly increased in hypothalamus in rats chronically exposed to emothinal stress. It was observed by in situ hybridization that the emotinal stress for 5days induced CRF m RNA expression only in parvocellulr neurons of paraventricular nucleus in rats. The exposure to emotional stress increased noradrenaline release of paraventricular nucleus examined by intracerebral microdialysis. The increase of noradrenaline induced by emotional stress for 5min on the first day of the experiment did not change when the same stress was repeated on the 7th day, which indicated that desensitization of noradrenaline neurons dose not occur. These changes induced by chronic exposure to emotional stress were smaller than those by electric footshock.The emotional stress used in our study induced the similar findings in rats to those in patients with anorexia nervosa except for hypogonadism, which was accompanied by activation of CRF and noradrenaline releases in paraventricular nucleus.