High Resolution Confocal Spinning Disc System

旋转圆盘共聚焦显微镜（SDC）是研究亚细胞动力学最成功的成像方法之一。基于相机的快速灵敏采集与共焦模式相结合，使研究人员能够在较长的时间内进行三维活细胞实验。AMBIO核心设施目前只有一个SDC系统，该系统已成功用于柏林各地研究小组的约80个项目。毫不奇怪，这个系统经常被超额预定，限制了活细胞实验的计划。高级功能的缺乏进一步阻碍了几个小组执行优化的成像项目。几个高端用户群体（即AGs Reber，Harms，Eickholt，Tacke，s。因此，要求更多使用容量和先进技术功能的研究组补充）是本提案的一部分，以采购一种新型SDC系统，该系统除了先前的功能（多路复用、自动化）外，还提供了提高吞吐量和分辨率的新功能（即大视场、组织成像光学器件、超分辨率）。除了这些小组之外，拟议的系统将提供给所有AMBIO用户（目前约42个研究小组中的约115个），以执行改进的高分辨率活细胞成像项目。

Spinning disk confocal (SDC) microscopy is one of the most successful imaging methods to investigate subcellular dynamics. The fast and sensitive camera based acquisition combined with a confocal mode enable researchers to perform multicolor 3D live-cell experiments over extended times. The AMBIO core facility currently has only one SDC system that has been successfully used in ~ 80 projects from research groups throughout Berlin. Not surprisingly, this system is frequently overbooked, limiting the planning of live-cell experiments. The lack of advanced features further prevents several groups from performing optimized imaging projects. Several high-end user groups (i.e. AGs Reber, Harms, Eickholt, Tacke, s. Supplement for Research Groups) who requested more usage capacities and advanced technical features are therefore part of this proposal to procure a novel SDC system that in addition to previous functionalities (multiplexing, automation) offers novel features (i.e. large field of view, optics for tissue imaging, super resolution) that enhance throughput and resolution. Beyond these groups the proposed system will be available to all AMBIO users (currently ~ 115 from ~ 42 research groups) to perform improved high-resolution live-cell imaging projects.