The analysis of ERECTA gene, which is responsible for the elongation of the stem, in Arabidopsis.

本研究的目的是：了解高等植物的发育过程，特别是拟南芥花茎的伸长过程。本研究的主要目的是分离突变体并分析相关基因。1.两个直立结合蛋白基因的研究。erbp 1和两个双突变体表现出幼苗致死性。通过对球形胚至成熟胚的观察，寻找个体发育缺陷点。这一缺陷在心脏期胚胎出现后才显露出来。2.为了寻找ERECTA基因表达调控的新因子，我们利用ER-uidA（来自E. coli）报道基因。我们分离的突变体携带直立（作为雷克斯）1和2基因的表达减少。它们的负责基因分别编码果胶合成和细胞器因子。在rexl和rex 2背景下测定相关基因的调控，了解其调控机制。

The aim of this study is: to understand the processes during the development of higher plants especially for the elongation of the floral stems in Arabidopsis. For that, our tools are to isolate mutants and to analyze the responsible genes.1.The study of two genes for erecta-binding proteins.The erbpl and 2 double mutants expressed seedling-lethality. The ontogenic defective point was searched by observation of the embryos from globular stage to maturity. The defect revealed after the onset of the heart-stage-embryo. The relation to auxin was determined by using auxin-reporter DR5-uidA.2.To search new factors for the regulation of ERECTA gene.We collected many mutants which have reduced expression of ER gene by using ER-uidA(from E. coli) reporter. We isolated mutants carrying reduced expression of erecta (as rex) 1 and 2 genes. Their responsible genes encode for pectin-synthesis and for factors for cellar organella, respectively. The regulation of related genes were determined under the rexl and 2 background to know the regulation.