Contribution of the Cortical Neuro-Glial Network to Memory Processing during Sleep

复杂运动任务的学习改变了初级运动皮层（M1）的局部网络活动，在随后的睡眠中基本上是排练的，并与记忆痕迹的区域间（即皮质-纹状体-海马）重组有关。在皮质水平，除了神经元，这一过程严重依赖于星形胶质细胞钙信号。然而，在随后的觉醒和睡眠过程中，参与记忆形成的协调神经胶质细胞活动的学习依赖性体内模式仍不清楚。使用慢性体内成像，我们将监测，在大脑状态的特定方式，神经元和星形胶质细胞的钙信号在M1之前，期间和之后立即复杂的车轮学习任务，以及在之前和之后的睡眠。我们将测试学习表现，潜在的任务特异性活动模式和区域间局部场电位（LFP）如何受到急性睡眠剥夺的影响，以及网络活动如何在类似但不相同的任务中重新激活，需要泛化过程，即，要点抽象总之，我们的数据将提供一个前所未有的洞察神经元和神经胶质细胞之间的体内相互作用的基础复杂的运动学习和它的排练睡眠。

Learning of complex motor tasks modifies local network activity in the primary motor cortex (M1), is substantially rehearsed during subsequent sleep and is associated with an interregional (i.e. cortico-striato-hippocampal) reorganization of the memory trace. At the cortical level, in addition to neurons, this process critically depends on astrocytic calcium signaling. However, the learning-dependent in vivo patterns of coordinated neuro-glial activity involved in memory formation during subsequent wake and sleep remain unclear. Using chronic in vivo imaging we will monitor, in a brain-state specific manner, neuronal and astroglial calcium signaling in M1 before, during and immediately after the complex wheel learning task as well as during preceding and subsequent sleep. We will test how learning performance, underlying task-specific activity patterns and interregional local field potentials (LFPs) are influenced by acute sleep deprivation and how network activity is re-activated post learning in a similar but not identical task, requiring generalization process, i.e., gist abstraction. Taken together our data will provide an unprecedented insight into the in vivo interactions between neurons and neuroglia underlying complex motor learning and its rehearsal by sleep.