Regenerating Cardiac Cells using GATA-4 Transfected Bone Marrow Stem Cells

我们先前已经报道了组织工程血管结构的成功创建和这些自体组织在肺循环中的47例临床植入。最近，一些研究机构开始在临床上使用基因治疗和细胞治疗来治疗缺血性心肌衰竭。本研究试图建立体外诱导骨髓基质细胞分化为心肌细胞的方法，并将其移植到体内模型中。为了从体外转染加塔-4基因的大鼠心肌细胞中获得搏动细胞，设计了两种不同的方案，方案A在共培养皿中检测来自大鼠心肌细胞的液体因子的作用，方案B在混合培养体系中检测细胞间的相互作用。将细胞片层系统应用于成人心肌细胞片层的制备。使用大鼠模型，应用Dr Nadal-Ginard方案（Mt Sainai，NY，USA），并且培养的干细胞将在培养物中生长。在获得足够数量的心脏干细胞后，将其植入同系大鼠缺血心脏模型中。所有实验仍在进行中。

We have previously reported the successful creation of tissue-engineered vascular structure and the 47 clinical implantation of these autologous tissue in the pulmonary circulation. Recently, several institutes started gene therapy and cell therapy in the clinical setting for the treatment of ischemic myocardial failure. In this study, we tried to establish the method to differentiate BMCs to myocardial phenotype in vitro, then implant to in vivo model. To obtain the beating cells in vitro from the GATA-4 transfected stormal cells in vitro, two differenct protocol was designed.In protocol A, the effects of liquid factors from neonatoal myocytes was examined in a co-culture dish.In protocol B, cell-to-cell interaction was examined using mixed culture system. The cell-sheet system will be applied to create adult cardiac cell sheet. Using a rat model, Dr Nadal-Ginard protocol (Mt Sainai, NY, USA) was applied and cultured stem cell will be grown in culture. After obtaining the sufficient number of cardiac stem cells, they will be implanted in a ischemic heart model of syngenic rat. All experiments are still underway.