Tissue Engineering for lower urinary tract by autologous bone marrow stem cell and adispose tissue

从临床角度看，在膀胱重建领域，尿路括约肌重建术对于预防尿失禁具有重要意义。首先，我们可以培养和扩增兔股骨骨髓间充质干细胞和腹部脂肪组织来源的干细胞。将培养的干细胞移植到损伤的尿路括约肌中，通过组织学方法(免疫组织化学、RT-PCR方法)和功能研究，可以找到重建的尿路括约肌。

From the clinical point of view, urethral sphincter reconstruction is important to prevent urinary incontinence in the field of bladder reconstruction. At first, we could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit and abdominal adipose tissue derived stem cells. The cultured stem cells were implanted into the injured urethral sphincter, and we could found the reconstruction of the urethral sphincter by histological methods (immunohistochemistry, RT-PCR method) and functional study.