Tissue Engineering for lower urinary tract by autologous bone marrow stem cell and adispose tissue

自体骨髓干细胞和脂肪组织用于下尿路的组织工程

• 批准号: 23592363
• 负责人: ISHIZUKA Osamu
• 金额: $ 3.33万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592363/
• 关键词: 移植・再生医療; 骨髄幹細胞; 脂肪細胞; 膀胱; 尿道括約筋; 脂肪幹細胞; 再生医学; 細胞・組織; 生体機能利用; 脳神経疾患

From the clinical point of view, urethral sphincter reconstruction is important to prevent urinary incontinence in the field of bladder reconstruction. At first, we could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit and abdominal adipose tissue derived stem cells. The cultured stem cells were implanted into the injured urethral sphincter, and we could found the reconstruction of the urethral sphincter by histological methods (immunohistochemistry, RT-PCR method) and functional study.

从临床角度来看，尿道括约肌重建是膀胱重建领域中预防尿失禁的重要手段。本研究首先从兔股骨来源的骨髓间充质干细胞和腹部脂肪组织来源的干细胞进行培养和扩增。将培养的干细胞植入损伤的尿道括约肌，通过组织学方法（免疫组化、RT-PCR方法）和功能研究，发现尿道括约肌的重建。

项目成果

Implantation of Autologous Bone-Marrow-Derived Cells Reconstructs Functional Urethral Sphincters in Rabbits

• DOI: 10.1089/ten.tea.2010.0478
• 发表时间: 2011-04-01
• 期刊: TISSUE ENGINEERING PART A
• 影响因子: 4.1
• 作者: Imamura, Tetsuya;Ishizuka, Osamu;Nishizawa, Osamu
• 通讯作者: Nishizawa, Osamu

Editorial commnet from Dr. Ishizuka to periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress incontinence: report of three initial cases.

Ishizuka 博士关于尿道周围注射自体脂肪再生细胞治疗男性压力性尿失禁的编辑通讯：三个初始病例的报告。

• DOI: 10.1111/j.1442-2042.2012.03145.x
• 发表时间: 2013
• 期刊: Int.J.Urol.
• 作者: Satoko Kojima;Takeshi Chiyomaru;Hideki Enokida;Kazumori Kawakami;Hirofumi Yoshino,Nijiro Nohata3;Miki Fuse;Tomohiko Ichikawa;Yukio Naya;Masayuki Nakagawa;and Naohiko Seki;Ishizuka O
• 通讯作者: Ishizuka O

The implantation of autologous adipose-derived cells makes the functional regeneration of frozen-injured rabbit urethra

自体脂肪细胞植入使冻伤兔尿道功能再生

• 发表时间: 2013
• 作者: Sudha;G.;Imamura;T.;Ishizuka;O.;Zhang;L.;Noguchi;W.;Yamagishi;T.;Yokoyama;H.;Kurizaki;Y.;Nishizawa;O.;Andersson;K.E.
• 通讯作者: K.E.

Implantation of autologous bone marrow-derived cells recovers urethral sphincter in rabbits

自体骨髓来源细胞植入恢复兔尿道括约肌

• 发表时间: 2011
• 作者: Imamura;T.;Ishizuka;O.;Kurizaki;Y.;Ishikawa;M.;Noguchi;A.;Zhang;L.;Ichino;M.;Kato;H.;Nishizawa;O.
• 通讯作者: O.

Implantation of autologous bone marrow -derived cells recovers urethral spinster composed of striated and smooth muscle layers in rabbits

自体骨髓来源细胞植入恢复兔尿道老处女由横纹肌层和平滑肌层组成

• 发表时间: 2011
• 作者: Imamura T;Ishizuka O et al.
• 通讯作者: Ishizuka O et al.