Regulation of Stem Cells Gene Expression using Apatite nanocarriers coated with Cell-reconizable chimeric Protein.

靶向基因及相关基因的DNA、RNA、蛋白质和药物的介导给药已成为国内外研究的热点。在这项研究中，我们首次显示了基于碳酸磷灰石的生物相容性无机纳米颗粒的基因和亲属的pH敏感性递送，其在典型的生理pH下高度稳定，并且在典型的内体室pH下快速降解。这种纳米尺寸（100- 300 nm）的磷灰石-基因缀合物显示出巨大的药物摄取到各种细胞，如通过荧光显微镜和流式细胞术所确定的。碳酸磷灰石纳米载体系统在干细胞（ES、EC细胞）中的应用成功地借助于细胞可电离的纳米生物材料如嵌合抗体以及载糖聚合物设计来完成。

Targeted genes and relatives DNA RNA protein and drug involved delivery has received much attention throughout the world. In this study, we show for the first time pH-sensitive delivery of gene and relatives based on biocompatible inorganic nanoparticles of carbonate apatite being highly stable at the typical physiological pH and quickly degradable at the typical pH of endosomal compartment. This nano sized (100-300nm) apatite-gene conjugates demonstrated huge uptake of drug to various cells as determined by Florescence microscopy and flow cytometry. The application of carbonate apatite nanocarriers systems to stem-cells (ES, EC Cells) are successfully done with aid of cell-recogonizable nano-biomaterials such as chimeric antibodies as well as sugar-carrying polymers design.