STRUCTURAL BIOLOGY ON MATURATION PROCESS OF METALLOPROTEINS

六种Hyp蛋白(HYPA、B、C、D、E和F)主要参与[NiFe]氢酶的成熟过程，催化可逆氢的产生和消耗。这些蛋白质将一个复杂的Ni/Fe簇结合到[NiFe]氢酶的一个蛋白质亚单位中。在我们首次成功测定HypC、HypD和Hype结构的基础上，我们对HYPA、HypB和HypF以及HypC、HypD和Hype之间的复合体进行了结构分析，以获得对[Nife]氢酶成熟的机制的见解。

Six Hyp proteins(HypA, B, C, D, E, and F) are primarily involved in the maturation process of[NiFe] hydrogenases which catalyze reversible hydrogen production and consumption. These proteins incorporate a complex Ni/Fe cluster into a protein subunit of[NiFe] hydrogenases. On the basis of our success in the first structure determination of HypC, HypD, and HypE, we have carried out here structure an alyses of HypA, HypB, and HypF and of complexes between HypC, HypD, and HypE to obtain mechanistic insights into the[NiFe] hydrogenase maturation.