Effect of oxidative stress on disulfide bond formation of uric acid efflux transporter BCRP

在这项研究中，我们研究了氧化应激对BCRP表达的影响，BCRP是一种尿酸外排转运蛋白。已知衰老诱导的氧化应激是由黄嘌呤氧化酶的活化引起的。因此，本研究选用黄嘌呤氧化酶作为底物。在Caco-2细胞中，黄嘌呤氧化酶的激活可以抑制BCRP S-S键的形成，而在HK-2细胞中则没有。这些结果表明，氧化应激对尿酸外排的影响在肠和肾之间是不同的。

In this study, we investigated that oxidative stress affected BCRP expression, which is a uric acid efflux transporter. It has known that aging-induced oxidative stress is caused by activation of xanthine oxidase. Thus, we used the substrates of xanthine oxidase in this study. In Caco-2 cells, the activation of xanthine oxidase was suppressed the BCRP S-S bond formation, but not in HK-2 cells. These results suggest that the effect of oxidative stress on the efflux of uric acid is different between intestine and kidney.