Identification of major host genes involved in the malaria parasite proliferation using mice.

这项研究的目的是在小鼠9号的Pymr1基因座上检测与疟疾寄生虫感染有关的宿主基因。(1)利用实时荧光定量聚合酶链式反应(Real-Time-PCR)分析，我们检测到疟疾易感小鼠的几个mRNA水平高于疟疾耐药小鼠。(2)利用小鼠BAC克隆建立了Tg小鼠品系，研究了该基因过表达对小鼠抗寄生虫感染能力的影响。使用这些转基因小鼠进行的敏感性分析仍在准备中。

The aim of this study was to examine host genes conferring resistance to infection with the malaria parasite in the Pymr1 locus on mouse Chr.9. (1) Using real-time PCR analysis, we detected that several mRNA levels were higher in malaria-susceptible mice than in malaria-resistant mice. (2) To examine the effects of the gene overexpression on resistance to infection with the parasite, we tried to generate TG mouse lines using mouse BAC clone. Susceptibility analyses using these TG mice are still in preparation.