5 laser flow cytometer for the detection of 20 parameters with high throughput sampling unit

根据这一提议，本杰明·富兰克林流式细胞仪设施(BFFC)申请增加一台能够进行多参数分析的流式细胞仪(18个荧光参数、2个散射探测器、5个激光器和一个高通量采样单元)。目前，柏林的Charité-Universityätsmediz本杰明·富兰克林(CBF)校区正在经历一次重大的扩张，并通过招聘新的实验室负责人及其小组在科学上重新焕发活力。在基础和临床研究中，迫切需要新的流式细胞仪在可能的最高水平上对稀有和/或复杂的细胞群体进行分析和功能表征(包括细胞周期分析和钙信号)。BFFC已经运行了类似的分析仪，但机器已经以最大容量运行，导致等待时间长，项目延误。Charité的其他流式细胞仪设施都没有提供具有5个激光的可比分析仪。多参数流式细胞术基本上是所有生物医学科学中的一项最先进的技术。特别是，临床样本的分析需要快速获得这样的分析仪，以保证对这些珍贵样本的及时分析。功能强大的高通量分析仪有助于快速分析多个样本和建立新的、强大的体外模型(例如，单细胞分化分析、有机物技术)。由于CBF最近招募了新的小组，迫切需要一种新的流式细胞仪来满足对多参数流式细胞术日益增长的需求。

With this proposal, the Benjamin Franklin Flow Cytometry Facility (BFFC) applies for an additional flow cytometry analyzer capable of multiparameter analysis (18 fluorescence parameters, 2 scatter detectors, 5 lasers and a high throughput sampling unit). Currently, the Campus Benjamin Franklin (CBF) of Charité - Universitätsmedizin Berlin undergoes a significant expansion and is being scientifically re-invigorated by the recruitment of new laboratory heads and their groups. The new flow cytometer is urgently needed for the analysis and functional characterization (including cell cycle analysis and Ca2+ signaling) of rare and/or complex cell populations on the highest possible level, both in basic and clinical research. BFFC already operates a similar analyzer but the machine already runs at maximal capacity leading to long waiting times and delays in projects. No other flow cytometry facility at Charité offers a comparable analyzer with 5 lasers. Multiparameter flow cytometry is a “state-of-the-art” technology in basically all biomedical sciences. In particular, the analysis of clinical samples requires rapid access to such an analyzer, in order to guarantee timely analysis of these precious samples. The powerful high-throughput analyzer facilitates rapid analysis of multiple samples and the establishment of novel and powerful in vitro models (e.g., single cell differentiation assays, organoid technology). Due to the recent recruitment of new groups at CBF a new flow cytometry analyzer is urgently needed to cope with the increasing demand for multi-parameter flow cytometry.