Clarification of the mechanism for acceleration of new bone formation by an electrically polarized hydroxyapatite from the molecular biological point of view

本文从分子生物学的角度阐明了极化羟基磷灰石对成骨细胞的作用，分析了极化羟基磷灰石对骨的影响，并利用微阵列技术对极化羟基磷灰石成骨相关基因进行了鉴定。由于微阵列的原因，同一样本之间存在差异，我们在Excel中以n=1为单位分析样本之间的差异，并整理数据。我们未来的挑战是利用微阵列软件，将同一样本中n=2差异较大的基因挑出并剔除，再次分析样本间的表达水平，缩小高表达基因的范围。我们希望通过微阵列综合筛选高表达基因，并通过实时PCR和westernblot对其进行分析，明确电极化HA表面与骨形成相关的基因。

By clarifying the osteoblastic cell behavior on polarized HA from the molecular biological point of view,we analized the effect on the bone of polarized HA and identification of gene concerning bone formation of polarized were done by microarray.As a result of microarray, there was a difference between same sample, we analyzed the differences between samples in n=1 in Excel and complied the data. Our future challenges are to use software for microarray, pick up and remove the genes that show big difference between n=2 in the same sample, and analyze the expression level between samples again to narrow down the high expression genes. We'd like to narrow down high expression genes by microarray comprehensively, analyze them by realtime PCR and westernblot, and clarify the genes relate to bone formation on the surface of electrically-polarized HA.