Joint Research on Microbial Enhancement Oil Recovery.

中国东北大学和吉林石油集团公司合作开展了一项微生物提高采收率（MEOR）工艺研究项目，旨在从地下油藏中开采更多原油。本研究的目的是通过实验室和实际油藏的实验来评价MEOR工艺的可行性。在中国扶余油田某水驱油藏进行了驱油方法的现场试验。用营养盐（糖蜜和无机化合物）注入菌株CJF-002。该菌株从扶余油田储层岩石中分离得到，经鉴定为Enterobacter sp.，具有产不溶性聚合物的能力，有望提高封堵高渗透层的波及效率。田间试验采用MPN-Direct-PCR法和荧光原位杂交（FISH）法进行快速检测。此外，还进行了仅注射营养盐或同时注射营养盐和细菌的田间试验。在一些不注射CJF-002菌株的试验中，储层中的天然微生物被激活，由于产液速度的增加，采油速度显著提高。

Tohoku University and Jilin Petroleum Group Co., China have been cooperatively proceeded a research project on microbial enhanced oil recovery (MEOR) processes in order to produce additional crude oil from oil reservoirs underground. The objective of this study was to evaluate the feasibility of the MEOR process through the experiment using both laboratory and actual oil reservoir. Field trials with flooding methods were carried out in a waterflooded reservoir of Fuyu-oilfield, China. Strain CJF-002 was injected to the reservoir with nutritive salts (molasses and inorganic compounds). The strain was isolated from reservoir rock of Fuyu-oilfield, identified as Enterobacter sp., and has an ability of producing insoluble polymer, which is expected to improve of sweep efficiency caused by plugging high permeability zone. MPN-Direct-PCR method and fluorescence in situ hybridization (FISH) method were used for rapid detection of the bacterium in the field trials. Field trials with huff-and-puff methods were also carried out with injecting nutritive salts only or with injecting nutritive salts and the bacterium. In some trials without injecting the strain CJF-002, natural microorganisms in the reservoir were activated and the oil production rate remarkably inceased due to the increase of the rate of fluid production.