Gametogenesis and production of offspring by the interspecific PGC transfer.

已经进行了PGCs的种间转移，并将转移的PGCs迁移到宿主动物的性腺组织中，但没有证据表明这些PGCs随后分化为功能配子。如果它们的PGCs具有功能，那么将它们的PGCs移植到一个更多产物种的胚胎中，就可以拯救濒临灭绝的物种。为了直接研究在受体动物体内转移的PGC，需要生殖细胞标记物来区分外源性和内源性来源的细胞。在本研究中，将分离的鹌鹑和PGCs移植到鸡胚中，并进行血管移植。进行了子代测试和精子DNA分析，以检查供体来源的配子。我们用三种单抗QCR1、QB2和2C9来区分鹌鹑和鸡的PGCs。QCR1和QB2为鹌鹑特异性单抗，2C9为鸡特异性单抗，具有发育特异性表达。转移的鹌鹑pgc在ch…定居。胚胎性腺较多，占性腺内生殖细胞总数的14.2%。一只输血的雄性幼体含有两个群体的精原细胞，分别来自外源性和内源性的雏鸡精原细胞。共移植了111枚雏鸡胚胎，其中22枚(19.8%)孵化成功，6枚雄性胚胎和2枚雌性胚胎成熟。在聚合酶链式反应分析中，在输血公鸡中观察到了来自QUAQUE-PGC的W染色体精子。转移的雏鸡PGCs在胚胎性腺中定居，占性腺中PGCs总数的5.6%。转PGC基因的雌性鹌鹑与公鸡杂交后的后代中，出现了单抗QCR1阳性的PGCs，表明这些杂交细胞是用公鸡精子受精后产生的杂交后代。在转PGC基因的雏鸡中，用聚合酶链式反应在7日龄胚胎、7日龄孵化的性腺组织和成熟雄性的精子中检测到鸡Z染色体特异性条带。此外，我们还设计了能区分鹌鹑和鸡基因组DNA的聚合酶链式反应引物，在嵌合鹌鹑精液DNA中检测到鸡基因组特异性条带。较少

Interspecific transfers of PGCs have been performed and the transferred PGCs migrated into gonadal tissues of the host animal, but there was no evidence that those PGCs differentiated into functional gametes thereafter. If their PGCs became functional, endangered species could be rescued by transferring their PGCs into the embryos of a more prolific species. In order to directly investigate the transferred PGCs in the recipient animal, germ cell markers are required that can distinguish between cells of exogenous and endogenous origins. In this study, isolated quail and PGCs were transferred into chick embryos, and vise vasa. A progeny test and an analysis of the sperm DNA were performed to examine the donor-derived-gamete. To distinguish between quail and chick PGCs, we evaluated three monoclonal antibodies (mAbs), QCR1, QB2 and 2C9. QCR1 and QB2 were quail-specific mAbs and 2C9 was a chick-specific mAb, showing development-specific expression. Transferred quail PGCs settled in the ch … More ick embryonic gonads and constituted 14.2% of total PGCs in the gonad. A transfused male hatchling contained two populations of spermatogonia, derived from exogenous quail and endogenous chick PGCs. Among 111 chick embryos transfused with quail PGCs, 22 (19.8%) hatched, and 6 males and 2 females reached maturity. In PCR analysis, quail-PGC-derived W-chromosomal sperm was observed in transfused roosters. Transferred chick PGCs settled in the quail embryonic gonads and constituted 5.6% of total PGCs in the gonad. Offspring from crosses between PGC-transferred female quail and roosters exhibited PGCs positively labeled with mAb QCR1, indicating that these were hybrids resulting from the fertilization of female quail ova by sperm of roosters. In chick PGC-transfused quail, the chicken Z-chromosome-specific band was detected by PCR in 7-day embryos, the gonadal tissues of 7-day hatchlings, and the spermatozoa of mature males. We have also designed PCR primers distinguishable between quail and chicken genomic DNA.Chicken genome specific band was observed in DNA of semen taken from chimeric quail. Less