Development of microinjection system

我们开发了用于处理DNA、细胞核、精子等的显微注射系统的自动化所需的元件技术，并将其与使用压电冲击驱动机构的微量吸管驱动系统相结合，安装在显微镜上，开发了用于显微授精的高效自动化显微注射系统。(1)细胞分选机构我们开发了一种细胞分选机构，利用波浪力将卵细胞从营养液中的任何位置聚集到显微镜视野的中心，这是通过摇动装满卵细胞的皿容器而发生的。(2)细胞旋转机制在去核手术中，要控制卵子的位置，同时考虑到核和极体的位置，这是一项难度很大的手术，对操作者的技能要求很高。因此，我们开发了细胞旋转机制， ...更多信息 旋转力来旋转和控制鸡蛋的位置。(3)基于图像处理技术的细胞定位系统在处理少量细胞的情况下，我们利用图像处理技术开发了一种将卵细胞定位在工作区中心的系统，并将上述机构与基于压电冲击驱动机构的微量移液器驱动系统相结合，制成了自动化显微注射系统的样机，并安装在倒置显微镜上。此外，还进行了利用静电力的细胞旋转机制的基础研究和实验，在小鼠卵细胞实验中，确认了受精后的发育率。在研究期间，对骏河精机公司开发的样机进行了运动试验、评价和检查。日本大学生物资源科学部、东海大学医学部和鹿儿岛大学农学部也将原型用于真实的细胞操作，并获得了许多建议和知识。少

We developed element technologies that are necessary for the automation of microinjection system that intended for handling DNA, a nucleus, sperm etc. And by combining them with the micropipette drive system using piezoelectric impact drive mechanism and installing them to a microscope, we developed a high efficiency automated microinjection system for micro insemination.The developed element technologies for microinjection system are these following three subjects.(1) Cell Sorter MechanismWe developed a Cell Sorter Mechanism for gathering egg cells from any position in the nutrient medium to the center of microscope view by using wave force, which occurred by shaking dish container that filled with egg cells.(2) Cell Rotation MechanismIn enucleating operation, to control the position of an egg which considered the position of the nucleus and the polar body is known as a difficult operation and requiring high skill of the operator. Hence, we developed cell rotation mechanism using elec … More trostatic force to rotate and control the position of the egg.(3) Cell Positioning System Using Image Process TechnologyIn case of dealing with a small amount of cells, we developed a system for positioning an egg cell in the center of work area by using image processing technology.We combined the mechanism mentioned above with the micropipette drive system using piezoelectric impact drive mechanism and made the prototype of the automated microinjection system, which installed to an inverted microscope. In addition, we also did fundamental researches and experiments of cell rotation mechanism using electrostatic force and in the experiments using mouse egg cells, the development rate after the fertilization was confirmed.In the research period, we did movement test, evaluation and examination on the prototype we developed in Suruga Seiki Co., Ltd. The prototype is also used for real cell operation in Nihon University College of Bioresource Sciences, Tokai University School of Medicine, and Kagoshima University Faculty of Agriculture, and got many advices and knowledge. Less