Spinning-Disk Microscope with optical Tweezer

理解控制生物系统功能的机械原理是现代研究的一个关键目标。细胞和组织力学的重要性是定量描述在发育和组织形成过程中以及在癌症中发生的动态过程的基础。我们使用光学镊子在单个细胞和组织中同时进行主动和被动微流变学。为了能够分配力的产生以及粘弹性性质的不同区域的粘附或有丝分裂细胞，也与胚胎损失以及癌症球体或重建的肌肉肌肉，这些必须识别与3D荧光技术。我们将耦合光学镊子在要求的旋转盘显微镜和镊子以及显微镜将由一个自编软件试点。除了微流变学，其他项目还将利用旋转盘显微镜的快速3D能力，使用2.5D牵引力显微镜测量单个细胞和单层的基底力。特别令人感兴趣的是由窄间隙产生的细胞在密闭条件下迁移的牵引力。利用嵌入单个细胞或整个组织中的弹性水凝胶珠，我们可以确定这些系统中的机械张力。这些弹性张力传感器有望对重建肌肉组织的运动和力量产生产生新的见解。

Understanding the mechanical principles governing the function of biological systems is a key goal of modern research. The importance of cell and tissue mechanics is fundamental for a quantitative description of the dynamical processes occurring during development and tissue formation but also in cancer. We use optical tweezers to perform simultaneous active and passive microrheology in single cells and tissue. In order to be able to assign the force generation as well as the viscoelastic properties to the different regions in adherent or mitotic cells, but also with embryonic loss as well as cancer spheroids or reconstituted muscle muscles, these have to be identified with a 3D fluorescence technique. We will couple an optical tweezer in the requested Spinning Disk microscope and the tweezers as well as the microscope will be piloted by a from self-written software. Besides microrheology, other projects will also make use of the rapid 3D capacity of the spinning disc microscope to measure substrate forces of individual cells and monolayers using 2.5D traction force microscopy. Of special interest are the traction force of cell migration in close confinement generated by a narrow gap. Using elastic hydrogel beads embedded in single cells or whole tissue, we can determine the mechanical tension in these systems. These elastic tension sensors promise new insights into the motion and force generation of reconstituted muscle tissue.