Role of renal arginase in acute renal failure

一氧化氮对细胞生理有双重作用。低浓度一氧化氮对血管系统有舒张作用。高浓度的一氧化氮对细胞有毒害作用。低浓度一氧化氮对急性肾功能衰竭患者的肾功能保护有重要作用。肾组织中不应产生高浓度的一氧化氮。我们研究了将精氨酸转化为NO底物的精氨酸酶；尿素和鸟氨酸。我们培养精氨酸酶II抗体，研究精氨酸酶II在肾脏中的定位。抗精氨酸酶II抗体强烈染色远端小管。胞浆呈颗粒状染色，提示心绞痛酶II可能是线粒体酶。这表明srginase II主要出现在急性肾功能衰竭中最脆弱的部位。到目前为止，还没有观察到明显的变化，可能是因为snginase II的变化很小。有必要通过更灵敏的方法，如激光捕获显微解剖来确定位置。在接下来的项目中，应该对这些方法进行研究。

Nitric Oxide has dual effects on cell physiology. Low concentration of Nitric Oxide has vasodilating effects on vascular system. High concentration of Nitric Oxide has toxic effects on cell. Low concentration of Nitric Oxide is useful for protecting renal function in acute renal failure. High concentration of Nitric Oxide should not be produced in renal tissue. We have investigated the arginase which convert arginine to substrate of NO ; urea and ornithine. We raised antibody to arginase II and investigated the localization of arginase II in the kidney. Distal tubule was strongly stained by anti-arginase II antibody. Cytosol was granularly stained in the cell, which suggests anginase II may be mitochondrial enzyme. This suggests that srginase II was mainly observed where are most fragile sites in the events of acute renal failure. So far, significant changes were not clearly observed, possibly because of the small changes of snginase II.It will be necessary to identify the locations by more sensitive methods such as laser capure microdissection. For the next projects, these methods should be investigated.