Factors involved in the interactions of Candida albicans and oral bacteria

通过监测生长培养基中的pH变化，研究了单一白色念珠菌分离株在唾液、血清包被或无蛋白质（未包被）、热循环的七种市售弹性内衬材料上的生长。使用三个参数观察组织调理剂对真菌生长的抑制作用，即：i）pH快速下降的开始延迟，ii）pH变化速率的降低和iii）达到的pH最小值。在对照软内衬（未经热循环处理且无涂层）的情况下，抗真菌作用似乎取决于所用市售软内衬的类型。因此，在湿固化和热固化有机硅材料中观察到pH下降的初始延迟和非常高的pH最小值。冷固化丙烯酸软内衬也观察到高pH值最小值，而冷固化硅胶材料与热固化丙烯酸树脂无显著差异（p>0.05）。然而，材料的抗真菌效果在热循环（ ...更多信息 ANOVA ; p<0.01）和蛋白质涂层（唾液，p<0.05 ;血清，p<0.01）。这些结果综合起来表明，材料的老化和宿主的生物液体，特别是血清，促进酵母菌在软衬里材料上的生长。在第二项研究中，对7种市售软衬材料（未涂层）进行了0、1000和10000次热循环（4 oC-70 oC，1 min），三磷酸腺苷（ATP）分析。在对照弹性内衬（未经热循环处理且无涂层）的情况下，真菌定植似乎取决于所使用的市售弹性内衬的类型。因此，除一种产品外，在热固化和热固化硅树脂材料、冷固化硅树脂材料中观察到最低的定植，热固化丙烯酸树脂表现出最高的定植能力，冷固化丙烯酸弹性内衬表现出中间水平。然而，热循环（ANOVA ; p<0.01）和蛋白质涂层（唾液，p<0.01 ;血清，p <0.01）都显著促进了材料上的真菌定殖。这些结果综合起来表明，材料的老化和宿主的生物流体促进了酵母菌在弹性衬里材料上的定殖。少

The growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled seven commercial resilient lining materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was observed using three parameters viz : i) delay in the onset of the rapid decline in pH, ii) reduction in the rate of pH change and iii) the pH minima reached. In the case of control soft liners (not thermocycled and uncoated), the antifungal effect appeared to depend upon the type of commercial soft liner used. Thus, an initial delay in pH decline, and very high pH minima were observed with fluoric and heat cured silicone materials. High pH minima were also observed with cold cured acrylic soft liners, whereas cold cured silicone materials, did not significantly differ from heat cured acrylic resin (p>0.05). However, the antifungal effect of the materials was significantly reduced both by thermal cycling ( … More ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.05 ; serum, p<0.01). These results, taken together, suggest that the ageing of the materials and the biological fluids of the host, particularly serum, promote yeast growth on soft lining materials.In the second study, the growth of a single isolate of C.albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4oC-70oC for 1min, respectively ; 0, 1000 and 10000 times) seven commercial soft lining materials were investigated, by adenosine triphosphate (ATP) analysis. In the case of control resilient liners (not thermocycled and uncoated), the fungal colonization appeared to depend upon the type of commercial resilient liner used. Thus, the lowest colonization was observed with fluoric and heat cured silicone materials, cold cure silicone materials, except for one product, and heat cured acrylic resin exhibited the highest colonization capacity, and cold cured acrylic resilient liners exhibited the intermediate. However, the fungal colonization on the materials was significantly promoted both by thermal cycling (ANOVA ; p<0.01) and a layer of protein coating (saliva, p<0.01 ; serum, p<0.01). These results, taken together, suggest that the aging of the materials and the biological fluids of the host promote yeast colonization on resilient lining materials. Less