Epidemiological investigation of protozoan diseases in China

1)原生动物寄生虫免疫优势抗原编码基因的克隆目前，许多原生动物寄生虫免疫优势抗原编码基因已被鉴定、克隆和鉴定。主要基因有：B.caballi P48、P134；武器装备EMA-1、EMA-2 P82; B。犬棘球绦虫P18、P29、P50，棘球绦虫SAG1、SRS2；弓形虫SAG1，SAG2,SRS1,ROP-1.2)建立原虫寄生虫抗体血清诊断方法将免疫优势抗原编码基因在大肠杆菌或昆虫细胞中表达，建立酶联免疫吸附试验、乳胶凝集试验和免疫层析试验。3)原生动物寄生虫检测聚合酶链反应的发展基于上述基因设计引物集，建立了原生动物寄生虫的PCR检测方法。4)中国原虫病流行病学调查对新疆、吉林、北京、福建和西藏4省的马、牛、猪、羊和狗的血液进行了巴贝斯虫病、弓形虫病、新孢子虫病和隐孢子虫病的诊断，获得了许多新的发现。

1)Cloning of genes encoding immunodominant antigens of protozoan parasitesIn the present study, many genes encoding irnmunodominat antigens of protozoan parasites were identified, cloned and characterized. The main genes are as follows : B.caballi P48, P134;B.equi EMA-1,EMA-2,P82;B.gibsoni P18,P29,P50;N.caninum SAG1,SRS2;T.gondii SAG1,SAG2,SRS1,ROP-1.2)Development of serodiagnostic methods for detection of antibodies to protozoan parasitesThe genes encoding immunodominant antigens were expressed in E.coli or insect cells, and then established the enzyme-linked immunosorbent assay, latex agglutination test, and immunochromatographic test with these recombinant antigens.3)Development of polymerase chain reactions for detection of protozoan parasitesThe primer sets based on genes described above were designed, and established PCR methods for detection of protozoan parasites.4)Epidemiological investigation of protozoan diseases in ChinaBlood samples collected from horses, cattle, pigs, sheep, and dogs, m Shinjiang, Jilin, Beijing, Fujian, and Tibet provinces, China, respectively, were examined for the diagnoses of babesiasis, toxoplasmosis, neosporosis, and cryptosrondiosis, and obtained many new findings.