Effect of neurotrophic factors on regeneration of denervated recurrent laryngeal nerve

在成年大鼠上观察了三种神经营养因子对失神经喉返神经(RLN)再生的影响。左侧RLN切断后，在神经残端之间放置硅胶管，神经间隙填充神经生长因子(NGF)、睫状神经营养因子(CNTF)、碱性成纤维细胞生长因子(BFGF)和磷酸盐缓冲液(PBS)作为对照。术后3周或5周进行肌电和组织学检查，局部应用NGF或bFGF可促进术后3周诱发肌电反应的恢复。CNTF和bFGF3周时可使再生神经纤维总数增加。应用碱性成纤维细胞生长因子可促进有髓神经纤维的恢复。神经生长因子和碱性成纤维细胞生长因子对延髓疑核运动神经元体积缩小有明显的预防作用。

Three kinds of neurotrophic factors were evaluated in adult rats for their effects on regeneration of the denervated recurrent laryngeal nerve (RLN). After transection of the left RLN, a silicone tube was placed between the nerve stumps, and the gap of the nerves was filled with one of the following solutions ; nerve growth factor (NGF), ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) and phosphate buffer (PBS) for control. Electromyographical and histological evaluations were conducted either 3 weeks or 5 weeks after the manipulation.Local administration of NGF or bFGF facilitated recovery of the evoked electromyographic response at 3 weeks. CNTF and bFGF increased the total number of regerating nerve fibers at 3 weeks. Recovery of the myelinated nerve fibers were facilitated by application of bFGF. NGF and bFGF were effective in the prevention of the decrease in the size of the motor neuron in the nucleus ambiguus due to transection.