Coevolutionary process between legume plants and parasaites/symbicants based on phylogenetic relationship.

本研究在国外研究的基础上，通过对种子捕食者（野童）和纹虫的分子系统发育分析，探讨了“资源消耗相互作用”网络的进化过程。我们考虑了两个假设过程：共同物种形成过程中的系统发育匹配和入侵过程中的宿主转移。(1)墨西哥野生蚕豆科昆虫与野豆科昆虫的相互作用(A)墨西哥野生蚕豆科昆虫与野豆科昆虫在墨西哥韦拉克鲁斯州、San-Lui Ptosi州和Quertaro州调查收集了野生蚕豆科植物的果实，记录了新兴野豆科昆虫的分子系统发育。虽然宏观上已经证实了Acacia-Mimosestes、Senna-Senius和Convolvlucae-Megacerus之间的系统发育关系，但微观上却显示出复杂的系统发育模式。(B)在亚洲栽培蚕豆和野童昆虫我们调查了台湾、泰国、缅甸和印度的蚕豆和野童昆虫之间的相互作用。系统发育分析表明，资源消耗交互模式可分为Vigna属和Phaseolus属。在室内实验中，研究了粗糙目昆虫的无聊行为和孵化幼虫的竞争类型。(2)Fabacae与Rhyzobium互作基于分子生物学和系统发育学分析了Fabacae与Rhyzobium的寄主特异性。在编码结瘤并决定宿主特异性的nod基因家族中，我们检测到了nodD基因。nodD是一种被多种次级代谢物质激活的转录因子，类黄酮复合物。由于它不能与其他类黄酮一起被激活，因此它决定了豆科和曲菌属的宿主特异性。

The present research investigates evolutionary processes of networks in "resource-consumer interaction", based on oversea research and molecular-phylogenetic analysis with seed-predator (bruchid insects) and Rhyzobium. Two hypothetical processes were considered : phylogenetic matching in cospeciation and host shift in invasion.(1)Fabaceae and bruchidae interaction(A)Wild Fabacae and bruchid insects in MexicoWe surveyed and collected wild fruits of Fabacae in Veracruz, San-Lui Ptosi, and Quertaro states in Mexico, and recorded emergent bruchid insect species with molecular phylogenetics. Although macro-scopic phylogenetic relationships have been confirmed between Acacia-Mimosestes, Senna-Senius, and Convolvlucae-Megacerus, micro-scopic showed complicated phylogenetic patterns with invasive host shift.(B)Cultivate Fabacae and bruchid insects in AsiaWe surveyed interactions between Fabacae and bruchid insects in Taiwan, Thailand, Myanmar, and India. The phylogenetic analysis shows that resource-consumer interactive patterns are divided into two genera Vigna and Phaseolus. Boring behavior and competitive types of hatched larvae in bruchid insects were investigated in laboratory experiments.(2)Fabacae and Rhyzobium interactionHost specificity was analyzed between Fabacae plants and Rhyzobium, based on molecular biology and phylogenetics. Among nod gene family that codes nodulation and determines host-specificity, we detected nodD gene. nodD is a transcription factor that is activated with various secondary metabolistic substances, flabonoid complex. Because it cannot be activated with other flabonoids, it determines host-specificity in Fabacae and Rhyzobium.