Development of a method for measurement of a time-weighted average concentration of polycyclic mutagens in marine environment-for practiced utilization in monitoring of marine environment.

(1)测定了日本内陆海中苯并(A)芘(BaP)的时间加权平均浓度(TWA)。1999年至2000年冈山沿岸采样点BaP的TWA浓度范围为0.16~8.77 ng/L，(2)水温对石膏球重量下降的影响。通过在流动水中悬挂石膏球的溶解试验，发现石膏球的重量下降与水流强度有很好的相关性，回归系数和回归线截距受水温的影响。我们得到了下面的公式：：C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)where C是水流强度(厘米/秒)，ΔW是石膏球的重量减量(G)，T是水温(℃)，因此，根据这个方程，它就可能成为…(3)挂起后蓝色人造丝的保存条件和BaP的回收率。将一纳克BaP溶解在烧杯中的蒸馏水中，让BaP吸附到BR上，然后计算在不同条件下保存后的BaP量与未保存的提取初始回收率。在-5℃下用铝箔包裹保存BR，1周和2周分别为101.6±8.2%和100.0±6.8%；在15-2O℃下用透明塑料薄膜包装保存4周，BR的保存率为93.5±5.1%。即使在室温下，BR对BaP的吸附量也保持在90%以上。本研究为样品的长途运输提供了一种方便实用的方法。(4)BR悬挂后样品中BaP含量与致突变性的关系。我们报道了用FHE-BR悬挂法检测到的样品的致突变性与BaP的含量呈正相关。这些现象大多是在流经城市的河流入海口悬挂的样品上观察到的。这一事实表明，除BaP外，城市污水中可能含有高度诱变物质。(5)研究的出版物和介绍。我们已在科学期刊和一些会议上发表和介绍了研究结果(见参考文献)。此外，我们正在准备在下列国际研讨会上作介绍，以便听取外国科学家的各种评价：(1)PRIMO11(2001年7月，英国普利茅斯)，(2)ISTA10(2001年8月，加拿大魁北克)，(3)8thICEM(2001年10月，日本静冈)。较少

(1) Results of measuring the time-weighted average (TWA) concentration of Benzo (a) pyrene (BaP) in the Seto Inland Sea, Japan.The values of the TWA concentration of BaP from 1999 to 2000 ranged from 0.16 to 8.77 ng/L for the sampling sites in Okayama seashore, from 0.08 to 7.04 ng/L for Osaka Bay sites and from undetectable level (0.01 ng/L) to 18.88 ng/L for the sites in Northern Kyushu.(2) Effects of water temperature on the decrease in weight of plaster balls.We gave a dissolving test by hanging a plaster ball in moving water and found that the decrease in weight of the plaster ball correlated well with water flow intensity, and the regression coefficient and the intercept of regression line were affected by water temperature. We obtained the following equation :C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)where C is water flow intensity (cm/sec), ΔW is the decrease in weight of plaster ball (g) and T is water temperature (℃) and Therefore, according to this equation, it becomes possibl … More e to correct the value of measured TWA if water temperature is known.(3) Preserving condition of blue rayon after hanging and the recovery rate of BaP.One nanogram of BaP was dissolved in distilled water in a beaker and let BaP adsorbed to BR.We then calculated the recovery rate from amount of BaP after keeping it under various conditions against the initial one that was obtained from extraction without preservation. They were 101.6±8.2% (for one week) and 100.0±6.8% (for two weeks) when BR was preserved with aluminium-foil-wrapping at -5℃, and 93.5±5.1% (for four weeks) when preserved with transparent-plastic-foil-wrapping at 15-2O℃. The amount of the BaP adsorbed to BR has maintained over 90% even in the room temperature. Our study provides a convenient and a practical procedure for long-distance transportation of the samples.(4) Relationship between the amount of BaP and mutagenicity in samples after BR hanging.We have reported that mutagenicity detected by fhe BR hanging technique positively correlated with the amount of BaP.The present study unexpectedly found some samples despite of small amount of BaP showed high mutagenicity. Most of these phenomena were observed on the samples hanging in estuaries of the rivers flowing through the cities. This fact suggests that the city sewages might contain highly mutagenic substances except for BaP.(5) Publications and presentation of the study.We have published and presented the study results in scientific journals and at some conferences (see references). Furthermore, we are preparing for presentations at following international symposiums in order to receive various evaluations from foreign scientists : (1) PRIMO11 (July 2001, Plymouth, UK), (2) ISTA10 (August 2001, Quebec, Canada), (3) 8thICEM (October 2001, Shizuoka, Japan). Less