Research for the establishment of artificial indoor breeding systems for the Japanese monkey using developmental technology

对日本猕猴(Macaca Fuscata)胚胎移植(ET)结合人工授精、体外受精(IVF)、卵胞浆内单精子注射(ICSI)、体外成熟(IVM)等生殖工程技术进行了四年(1999-2002年)的研究，结果如下：(1)建立了从心电图(PMSG)中获取成熟卵子的方法；(2)建立了将2-4细胞胚胎移植到输卵管的体外受精-胚胎移植(IVF-ET)方法。(3)利用该方法成功地获得了世界上第一例体外受精-胚胎移植(IVF-ET)日本猴的活产。(4)建立了电刺激或直接从安乐死受试者附睾液中获取精子的液氮冷冻方法。(5)建立了冷冻精子ICSI-ET方法。利用该方法成功培育出世界首例食蟹猕猴胚胎。(6)建立了ZYGO…更多的TE IVM方法。利用这种方法在日本猕猴和食蟹猕猴身上成功建立了世界上第一个胚胎干细胞(ES细胞)。(7)突破了液氮合子冷冻保存的接近。(8)截至今天，已有9只人工生产的个体断奶。截至2002年3月，已确认人工受孕28次。(9)所有人工生产的个体均为沙门氏菌、志贺氏菌、结核病、内部寄生虫、外部寄生虫、B病毒、HSV-1、SIV、SRV、SVV、丝状病毒、STLV和麻疹抗体阴性。现在生产无特定病原体(SPF)的个体成为可能。(10)成功生产ISCI-ET日本猴子仍然难以捉摸：必须找出原因。目前在日本猴子上建立的以下方法：使用腹腔镜从刺激的卵巢收集卵子；ICSI；使用腹腔镜的输卵管ET；精子和受精卵的冷冻保存。使用这些方法进行有计划的室内繁殖日本猴子所需的所有准备工作据信都已完成。较少

Results of four year (1999-2002) investigation of embryo transfer (ET) in the Japanese monkey (Macaca fuscata) in association with reproductive engineering techniques such as artificial insemination, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), in vitro maturation (IVM), etc., are as follows :(1) Established method for retrieval of mature ova from eCG(PMSG), hMG and FSH stimulated ovaries using laparoscopy.(2) Established IVF-ET method for transfer of 2-4 cell embryo to the fallopian tube under laparoscopic observation.(3) Successfully produced world's first IVF-ET pregnancy and live birth in the Japanese monkey using this method.(4) Established liquid nitrogen cryopreservation method for sperm collected either by electrostimulation or directly from euthanized subjects' epididymis.(5) Established cryopreserved sperm ICSI-ET method. Successfully produced world's first ICSI-ET crab eating macaque (Macaca fascicularis) fetus using this method.(6) Established zygo … More te IVM method. Successfully established world's first embryonic stem cell (ES cell) in the Japanese monkey and the crab eating macaque using this method.(7) Breakthrough in liquid nitrogen zygote cryopreservation close.(8) As of today nine artificially produced individuals weaned. As of March, 2002, 28 artificial impregnations confirmed.(9) All artificially produced individuals confirmed negative for salmonella, shigella, tuberculosis, internal parasites, external parasites, B-virus, HSV-1, SIV, SRV, SVV, filovirus, STLV, and measles antibodies. Production of specific pathogen free (SPF) individual now possible.(10) Success producing a ISCI-ET Japanese monkey remains elusive : must identify reason why.Following methods now established in the Japanese monkey : ova collection from stimulated ovary using laparoscopy ; ICSI ; fallopian tube ET using laparoscopy ; sperm and fertilized zygote cryopreservation. All preparations necessary for planned, indoor breeding of the Japanese monkey using these methods believed complete. Less