Control of acyl-CoA dehydrogenase expression by androgen.

金仓鼠哈德氏腺分泌丰富的脂质，主要由1-烷基- 2,3 -二酰基甘油（ADG）组成，其组成具有明显的性别差异。男性腺体含有直链脂肪酸，而女性腺体另外含有支链脂肪酸。我们发现这些差异是由雄激素调节酰基辅酶a脱氢酶引起的，而酰基辅酶a脱氢酶是支链脂肪酸形成的关键酶。通过薄层色谱（TLC）分析ADG来检测性别差异。即雄性ADG谱为3个点，雌性ADG谱为2个点。利用该腺体的特点，我们通过分析腺体分泌液的ADG特征来监测雄激素和抗雄激素物质的作用。阉割的雄性仓鼠（术后2周）皮下注射含或不含氟他胺等抗雄激素的睾酮。用玻璃毛细管从每只动物身上提取约1 μ1的腺液，持续2周。阉割后薄层色谱上ADG谱变为雌性型。睾酮治疗使其恢复到男性类型，时间和剂量依赖。然而，当额外注射抗雄激素时，这些变化被消除。抑制作用也依赖于抗雄激素的剂量。这些结果清楚地表明，我们可以很容易地利用动物无创地评估雄激素和抗雄激素材料的作用。

The Harderian gland of golden hamster secretes copious lipids consisting mainly of 1-alkyl-2, 3-diacylglycerol(ADG), the composition of which shows marked sexual differences. Male gland contains straight chain fatty acids, while female gland additionally contains branched chain ones. We found these differences were caused by the androgenic regulation of acyl-CoA dehydrogenases which are the key enzymes for the formation of branched chain fatty acids. The sexual differences were detectable by analyzing ADG on TLC.Namely, ADG profile was composed of 3 spots in male while 2 spots in female. Utilizing the characteristics of this gland, we monitored the effects of androgenic and anti-androgenic materials by analyzing ADG profile of secreted fluid from the gland. Castrated male hamsters(2 weeks after operation)were subcutaneously injected with testosterone with or without anti-androgens such as flutamide. We obtained about 1 μ1 of gland fluid with a glass capillary from each animal for 2 weeks. ADG profile on TLC changed into female type by castration. Testosterone treatment returned he profile to male type time- and dose -dependently. However these changes were abolished when anti-androgens were additionally injected. The inhibition was also dependent on the dose of anti-androgens. These results clearly show that we can easily evaluate the effects of androgenic and anti-androgenic materials using animals noninvasively.