Regulation of meiotic chromosome segregation by cohesin Rec8

与高等真核生物一样，裂变酵母着丝粒由重复的DNA结构和相关的异染色质蛋白质复合物组成，其在细胞分裂期间染色体的忠实分离中起关键作用。对于姐妹染色单体的凝聚和正确的染色体分离至关重要的凝聚素蛋白复合物在着丝粒重复区富集。我们已经确定了异染色质和粘着蛋白之间的功能和物理联系。我们发现，在着丝粒重复的粘附素的优先本地化是依赖于Swi 6，一个保守的异染色质蛋白，是需要适当的动粒，功能。在交配型异染色质区也富含粘着蛋白，这是Swi 6依赖性的，并且是保持该基因座基因组完整性所必需的。我们提供的证据表明，粘附素亚基Psc 3与Swi 6及其小鼠同源物HP 1相互作用。这些数据定义了一个保守的功能Swi 6/HP 1在招聘的凝聚异染色质区域，促进染色体的正确分离。

Fission yeast centromeres, like that of higher eukaryotes, are comprised of repeated DNA structures and associated heterochromatin protein complexes, that play a critical role in faithful segregation of chromosomes during cell division. Cohesin protein complexes, which are essential for sister chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats. We have identified functional and physical link, between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore, function. Cohesin is also enriched at the mating type heterochromatic region in a Swi6 dependent manner and is required to preserve genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homolog HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting proper segregation of chromosomes.