Exploitation of the new method for the identification of baleen whales by using the SINE method

为了找到对物种鉴定有用的基因座，我们需要以下步骤：1）筛选鲸类基因组文库2）SINE侧翼区序列测定3）设计能扩增SINE基因座的寡核苷酸引物4）以几种鲸类的基因组DNA为模板进行PCR 5）寻找物种特异性插入西内斯的基因座。在这项研究中，我们已经确定了几个位点，这是有用的物种鉴定须鲸。它们是以下，须鲸特有的;驼峰20，驼峰203，驼峰9。驼峰14，驼峰30，驼峰34，驼峰52，驼峰55，鳍6，鳍11，鳍17，Sei 8-2，Sei 10-1，Sei 24，Sei 25，Sei 29-2，Mnk 15，Mnk 25，Nag 13，Nag 23，BRY 15，BRY 18，BRY 27，BRY 44，BRY 48，BRY 50，BRY 54，Blu 2，Blu 7，蓝22蓝27蓝78长须鲸特有的;鳍17，鳍18，Nag 18。座头鲸特有;驼峰13，驼峰33，驼峰42，驼峰57，鳍9。布氏鲸特异性; BRY 63.小须鲸专用; Mnk 1、Mnk 30、Mnk 31、NM 35、SMK 34、KIT 90。对于目前无法鉴定种类的鲸类，我们将继续进行进一步的实验。

To find the loci which are useful for species identification, we need the following procedures ; 1) Screening the genomic libraries of cetaceans 2) Sequencing of SINE flanking region 3) Designing the oligonucleotide primers that can amplify the SINE locus 4) Performing the PCRs by using the genomic DNAs of several cetacean species as templates 5) Find the loci to which SINEs are inserted species specifically. In this research, we have characterized several loci, which is useful for species identification of baleen whales. They are the followings, Baleen whales specific ; Hump 20, Hump 203, Hump 9. Hump 14, Hump 30, Hump 34, Hump 52, Hump 55, Fin 6, Fin 11, Fin 17, Sei 8-2, Sei 10-1, Sei 24, Sei 25, Sei 29-2, Mnk 15, Mnk 25, Nag 13, Nag 23, BRY 15, BRY 18, BRY 27, BRY 44, BRY 48, BRY 50, BRY 54, Blu 2, Blu 7, Blu 22, Blu 27, Blu 78. Fin whale specific ; Finl7, Fin 18, Nag 18. Humpback whale specific; Hump 13, Hump33, Hump 42, Hump 57, Fin 9. Bryde's whale specific ; BRY 63. Minke whale specific ; Mnk 1, Mnk 30, Mnk 31, NM 35, SMK 34, KIT 90. Concerning the cetaceans for which the species identification is not available now, we continue the further experiments.