Studies on the evaluation and development of Colombian Medicinal Plants

在项目期间，我们三次访问哥伦比亚，深入波哥大、波哥大郊区、哥伦比亚亚马逊地区莱蒂西亚的当地市场，获取了大量民间药用植物资料，制备了36种药用植物的甲醇提取物样品进行评价。在盐水对虾毒力生物测定筛选中，紫棘、巨心和薇甘菊种子提取物均表现出毒性活性。在以清除DPPH自由基为检测指标的抗氧化活性评价中，印加和树麻叶提取物表现出较强的抗氧化活性。在抑制HCV RNA聚合酶的实验中，发现香柏木和巨心木种子提取物有较好的抑制效果。在研究植物提取物增强抗生素抗MRSA作用的协同效应时，发现线虫叶/茎提取物、山楂叶提取物和美洲铜皮提取物具有显著的抗MRSA活性。生物测定引导分离提取物使我们能够表征一些有效成分。在这些药用植物提取物中，我们分离并确定了15种新物质的结构。

During the term of project we visited Colombia three times and stepped into local markets in Bogota, suburb areas of Bogota and Leticia in Colombian Amazon, and obtained a number of information on folk medicinal plants and prepared methanol extract samples of 36 medicinal plants for evaluations. In the screening of brine shrimp lethality bioassay, the extracts of Spondias purpurea seeds, Anacardium giganteum seeds and Mikania glomerata showed the toxic activity. In the evaluation of anti-oxidant activity monitored by DPPH radical scavenging effects, leave extracts of Inga spectabilis and Campomanesia lineatifolia displayed potent activity. In the assay of the inhibition of HCV RNA polymerase, extracts of Cedrela odorata burk and Anacardium giganteum seeds found to be effective. In the search of synergistic effect of plant extracts to enhance the action of antibiotics against MRSA, the extracts of Matayba elegans leaves/stem, Campsiandra commosa burk and Cupania americana aerial part exhibited significant activity. Bioassay-guided separation of the extract allowed us to characterize the some of the active constituents. Among these medicinal plant extracts we have isolated and determined the structures of 15 new substances.