Perfect prevention of oxidation of material with biological activity using regeneration reaction of lipid soluble antioxidant with catechin

本研究的目的是评价儿茶素的抗氧化活性，并构建一种新的方法，用于防止实际食品系统中具有生物活性的所需物质的氧化，使用脂溶性抗氧化剂与儿茶素的再生反应。2002年，在惰性正癸烷溶剂中，加入表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）或表儿茶素没食子酸酯（epicatechin gallate，ECG），进行了β-胡萝卜素的氧化实验。在这两种情况下，观察到诱导期，其中β-胡萝卜素浓度略有下降，氧化被抑制。诱导期随ECG浓度的增加而延长，但在一定浓度下诱导期达到最大值。已知含有吡罗没食子酸基团的EGCG自身氧化以产生活性氧。因此，EGCG被认为具有渐进的氧化活性以及抗氧化活性。 ...更多信息 当用作食品添加剂时溶解在水包油乳液中。儿茶素具有两亲性，更易溶于水相。β-胡萝卜素和脂溶性抗氧化剂α-生育酚与儿茶素的反应发生在油-水界面上。2003年，在具有一定界面面积的两相油水体系中进行了α-生育酚存在下的氧化实验。在两相体系中，α-生育酚的保留时间比在正癸烷单相体系中长。由于α-生育酚在油相中的存在抑制了β-胡萝卜素的氧化，因此水相的存在对抑制β-胡萝卜素的氧化是有效的。α-生育酚被氧化消耗，β-胡萝卜素的抗氧化保护作用也被消耗。α-生育酚的过氧自由基与链氧化反应有关，易溶于水。这种现象可能有助于抑制α-生育酚的消耗，从而抑制β-胡萝卜素的氧化。在考虑β-胡萝卜素氧化和抗氧化机理的基础上，考虑α-生育酚过氧自由基向水相中的转移，建立了一个动力学模型。该模型定量描述了α-生育酚在较宽初始浓度范围内的氧化行为。少

The purpose of this research is to evaluate the antioxidant activity of catechin and to construct a novel method for preventing the oxidation of desired materials with biological activity in the actual food system using a regeneration reaction of lipid soluble antioxidant with catechin. In 2002, the oxidation experiments of β-carotene, an active oxygen quencher, were performed in an inert n-decane solvent with addition of epigallocatechin gallate (EGCG) or epicatechin gallate (ECG). In both cases, the induction period, in which the β-carotene concentration decreased slightly, was observed and the oxidation was suppressed. The induction period became longer with increasing the ECG concentration, whereas the period had a maximum at a certain EGCG concentration. EGCG containing pirogallol group is known to oxidize itself to produce activated oxygen. Therefore, EGCG was considered to have a progressive activity of the oxidation as well as the antioxidant activity.β-Carotene is commonly dis … More solved in the oil in water emulsion when used as a food additive. Catechin haying amphiphilic property is more easily dissolved in the water phase. The reaction of β-Carotene and lipid-soluble antioxidant, α-tocopherol, with catechin occurs on the oil-water interface. In 2003, the oxidation experiments in the presence of α-tocopherol were performed in the biphasic oil-water system having a definite interfacial area. In the biphasic system, the time for which α-tocopherol remained was longer than that in the single phase of n-decane. Since the β-carotene oxidation was suppressed during a-tocopherol remained in the oil phase, the existence of water phase was effective for the suppression of β-carotene oxidation. α-Tochopherol was consumed by its oxidation as well as the antioxidative protection of β-carotene. The peroxyl radical of α-tocopherol concerned with the chain oxidation was easy to dissolve in the water. This phenomenon seemed to contribute the restrain of α-tocopherol consumption, resulting in the suppression of β-carotene oxidation. A kinetic model was constructed by considering the transfer of the α-tocopherol peroxyl radical into the water phase in addition to the oxidation and antioxidation mechanisms of β-carotene. The model quantitatively described the oxidation behavior of β-carotene in the biphasic system over a wide range of the initial α-tocopherol concentrations. Less