Study of effect of host nicotine metabolism on progress of periodontitis in smokers

(The 2001财政年度）比较了吸烟者和非吸烟者的牙周疾病的疾病状态与唾液中潜血反应试验结果之间的关系，并通过测量尼古丁的代谢物可替宁的唾液浓度来检验。结果表明，吸烟组中隐血阴性者较多，牙周炎虽有进展，但唾液中可替宁浓度高的组牙周炎有加重的趋势。在牙周炎进展组中，唾液隐血反应试验与唾液中可替宁浓度呈显著负相关，唾液隐血量随着唾液中可替宁浓度的升高而降低。(The 2002财政年度）作为体液中可替宁和尼古丁的定量方法，旨在开发高效液相色谱法 ...更多信息 色谱法（HPLC）。通过改变色谱柱和移动的相，采用不同的洗脱条件，对尼古丁和可替宁的超灵敏检测条件进行了优化，建立了尼古丁和可替宁的线性定量方法，其线性范围分别为94 ~ 2350 ng/ml和122 ~ 3050 ng/ml。然而，该检查对唾液组分不利，因为与可替宁峰重叠的物质混合。当样品变更为血清时，可以确认没有阻碍可替宁和尼古丁含量的峰，并且可以很好地进行分析。(The对某保健中心413名体检者进行了牙周健康调查评估结果与吸烟量的各项关系的调查，并采用高效液相色谱法测定了血液中可替宁的含量。问卷调查的吸烟量和HPLC定量测定的可替宁含量与牙周炎的进展有显著相关性。同时，问卷调查显示吸烟量较多但血中可替宁含量不高的受试者，牙周炎有进展的趋势。也就是说，在尼古丁代谢较低的人中，是毒性持续影响，并表明牙周炎进展的可能性。少

(The 2001 fiscal year)The relationship between disease state of periodontal disease and occult blood reaction test result in the saliva in the smoker and nonsmoker was compared and was examined by the measurement of the saliva concentration of the cotinine which is a metabolite of the nicotine. As the result, there were many occult blood negative subjects in the smoking group, though periodontitis progresses, and the periodontitis tended to make it into the seriousness in the group of which the cotinine concentration in the saliva was higher. In the group in which the periodontitis progressed, there was the significant inverse correlation between saliva occult blood reaction test and cotinine concentration in the saliva, and the saliva occult blood quantity lowered with the rise in the cotinine concentration in the saliva.(The 2002 fiscal year)As a quantitative method of cotinine and nicotine in body fluid, it aimed at the development of the method using high performance liquid chiroma … More tography(HPLC). As a result of retrieving the condition for supersensitively detecting nicotine and cotinine by the change of column and mobile phase variously using various elution conditions, the method for obtaining the rectilinear quantitativity for nicotine and cotinine was able to be developed in 94〜2350ng/ml and in 122〜3050ng/ml, respectively. However, this inspection was not good for the saliva component, because the material which overlapped with the peak of the cotinine intermingled. It was able to be confirmed that there was no peak which obstructs quantity of cotinine and nicotine and that the analysis can be carried out well, when the sample was changed to be the serum.(The 2003 fiscal year)Medical examinees of 413 persons of the some health care center were examined on each relation of assessment result of periodontal health survey and smoking quantity according to the questionnaire vote and cotinine quantity in the blood by HPLC. The significant correlation as the result at smoking quantity according to the questionnaire vote and cotinine quantity determined quantitatively by HPLC and progress of periodontitis was recognized. In the meantime, in the subjects whose smoking quantity was abundant according to the questionnaire vote but cotinine quantity in the blood was not high, periodontitis tended to advance. That is, in the person whoes metabolism of the nicotine is lower, it was that the toxicity continuously affects, and the possibility that the progress of periodontitis advances was indicated. Less