CONSTRUCTION AND APPLICATION OF CDNA MICROARRAY SPECIALIZED FOR PERIODONTAL IIGAMENT

1.人牙周膜基因表达谱的分析我们用人牙周膜（PDL）的3 -directional cDNA文库收集序列，它忠实地代表了mRNA群体的组成。利用这些信息，我们研究了活性基因的表达谱。我们发现PDL具有独特的活性基因表达谱.一个新基因PLAP-1的鉴定和鉴定通过分析人PDL的a基因表达谱，我们鉴定了一个新基因，它编码一个新的富含小let cine重复序列的蛋白聚糖家族成员。我们将其命名为PLAP-1（牙周膜相关蛋白-1）。PLAP-1在体内PDL中特异性表达，在维持PDL的稳态中起重要作用.人牙周膜特异性cDNA微阵列的构建选择人牙周膜中表达的基因，我们构建了人牙周膜特异性cDNA微阵列， ...更多信息 我们命名为PerioGen Chip，PerioGen Chip包含从人PDL的3 -定向cDNA文库获得的1，600个cDNA克隆。利用PerioGen芯片分析PDL细胞在体外分化过程中的基因表达模式。PerioGen芯片分析鉴定出15个基因在细胞分化过程中表现出两倍或更多的差异表达。在这些基因中，我们重点研究了DAN基因和Periostin基因，这两个基因表现出了较强的转录诱导作用。我们证明了DAN和Periostin在调节PDL细胞的细胞分化中起着至关重要的作用.牙周病患者牙周组织的基因表达分析。我们能够确定主要在牙周病变中表达的几个基因。这表明PerioGen芯片可以用于临床应用。少

1. Analysis of gene expression profile of human periodontal ligamentWe collected sequences with the 3 -directed cDNA library of human periodontal ligament (PDL), which faithfully represents the composition of the mRNA population. Utilizing the information, we investigated the expression profile of the active genes. We revealed that PDL has a unique active gene expression profile.2. Identification and characterization of a novel gene, PLAP-1By analyzing the a gene expression profile of human PDL, we identified a novel gene, which encodes a new member of the small let cine-rich repeat proteoglycan family. We designated it PLAP-1(periodontal ligament associated protein-1). It was shown that PLAP-1 is expressed specifically in PDL in vivo and plays an important role in maintaining homeostasis of PDL.3. Construction of customized cDNA microarray specialized for human PDLSelecting genes that are expressed in human PDL, we constructed in-house cDNA microarrays specialized for human PDL, which … More we named PerioGen Chip PerioGen Chip contained 1,600 cDNA clones obtained from the 3 -directed cDNA library of human PDL.4. Gene expression analysis of PDL utilizing PerioGen Chip Utilizing PerioGen Chipwe analyzed the patterns of gene expression during the cytodifferentiation of PDL cells in vitro. PerioGen Chip analysis identified 15 genes showing twofold or more differential expression during the cytodifferentiation. Among these genes, we focused on DAN gene and Periostin gene which showed strong induction of their transcript. We demonstrated that DAN and Periostin have crucial roles to regulate cytodifferentiation of PDL cells.5. Gene expression analysis of in vivo periodontal tissues by PerioGen ChipWe performed gene expression analysis of periodontal tissues which were derived from patients with periodontal diseases. We were able to identify several genes which were expressed predominantly in periodontal lesions. This demonstrated that PerioGen Chip can be useful for clinical application. Less