Complexity single cell analysis designed to create a new periodontal regenerative medicine in silico

实时活细胞成像显示成纤维细胞生长因子-2促进了细胞周期和细胞分裂。此外，成纤维细胞生长因子-2刺激牙周膜细胞在Will愈合模型中迁移。在该模型中，血管内皮细胞生长因子进一步增强了成纤维细胞生长因子-2诱导的迁移。内皮细胞与牙周膜细胞共培养可促进内皮细胞管腔的形成，这种作用部分是由成纤维细胞生长因子-2刺激的牙周膜细胞产生的血管内皮生长因子引起的。这些结果至少部分揭示了成纤维细胞生长因子-2促进牙周再生的机制。

Real-time live cell imaging revealed that FGF-2 promoted the cell cycle and the division of the cells. Furthermore, FGF-2 stimulated migration of periodontal ligament cells in a would healing model. In this model, VEGF further enhanced the FGF-2-induced migration. It was also demonstrated that co-culture of endothelial cells with periodontal ligament cells stimulated the lumen formation of the endothelial cells and this effect was partially caused by VEGF produced by FGF-2-stimulated periodontal ligament cells. These results revealed, at least in part, the mechanisms of periodontal regeneration by FGF-2.