Establishment of periodontal tissue engineering by FGF-2

FGF-2建立牙周组织工程

基本信息

  • 批准号:
    14370709
  • 负责人:
  • 金额:
    $ 8.58万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2004
  • 项目状态:
    已结题

项目摘要

We investigated the regulatory effects of basic fibrovlast growth factor(bFGF) on the production of various extracellular matrices which play important roles in the regulation of periodontal tissue regeneration and can be candidate(s) of the carrier material(s) of bFGF-containing regenerative medicine. It was demonstrated that bFGF preferentially induced hyaluronan and heparan sulfate production by human periodontal ligament (HPDL) cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of bFGF-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed that the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS2, both of which contribute to the production of HA with a high molecular mass. In terms of heparan sulfate, the shedding of syndecan-2 of the surfaces of bFGF-treated HPDL cells was observed. The transcriptome analysis of HPDL cells revealed the existence of periodontal ligament associated protein-1 (PLAP-1), novel proteoglycan-like protein. We demonstrated that PLAP-1 negatively regulated the cytodifferentiation of HPDL cells into hard-tissue forming cells and that bFGF downregulated the expression of PLAP-1. In terms of bone-related proteins, bFGF downregulated the expressions of bone sialoprotein, osteonectin and osteocalcin by HPDL cells. Interestingly, however, bFGF clearly enhanced the osteopontin expression at both mRNA and protein levels. The osteopontin was detected in the cytoplasm and the conditioned medium but not on the surfaces of bFGF-treated HPDL cells. Furthermore, it was revealed that ERK1/2 and PKC were involved in the signaling pathway(s) of bFGF-induced osteopontin expression. We are now planning to investigate the efficacy of bFGF plus scaffold material(s) to induce periodontal tissue regeneration by utilizing the severe periodontitis model of beagle dogs.
我们研究了碱性成纤维细胞生长因子(bFGF)对各种细胞外基质产生的调节作用,这些细胞外基质在牙周组织再生的调节中起重要作用,并且可以是含bFGF的再生医学的载体材料的候选者。结果表明,碱性成纤维细胞生长因子优先诱导透明质酸和硫酸乙酰肝素的人牙周膜(HPDL)细胞的生产在剂量依赖性的方式。HPLC分析显示,与未处理的HPDL细胞相比,在bFGF处理的HPDL细胞的条件培养基中,HA具有更高的分子量。RT-PCR分析显示,透明质酸合成酶(HAS)1和HAS 2的mRNA表达增强,这两个基因都有助于产生高分子量的HA。就硫酸乙酰肝素而言,观察到bFGF处理的HPDL细胞表面syndecan-2的脱落。HPDL细胞的转录组分析显示存在牙周膜相关蛋白1(PLAP-1),一种新的蛋白多糖样蛋白。我们证明,PLAP-1负调控HPDL细胞分化成硬组织形成细胞和bFGF下调PLAP-1的表达。在骨相关蛋白方面,bFGF下调HPDL细胞骨涎蛋白、骨粘连蛋白和骨钙素的表达。然而,有趣的是,碱性成纤维细胞生长因子在mRNA和蛋白质水平上明显增强了骨桥蛋白的表达。bFGF处理的HPDL细胞的细胞质和条件培养基中检测到骨桥蛋白,但不在细胞表面上。此外,还发现ERK 1/2和PKC参与bFGF诱导骨桥蛋白表达的信号通路。我们目前正计划利用比格犬的重度牙周炎模型来研究bFGF加支架材料诱导牙周组织再生的功效。

项目成果

期刊论文数量(40)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effects of basic fibroblast growth factor on human gingival epithelial cells
  • DOI:
    10.1902/jop.2002.73.12.1467
  • 发表时间:
    2002-12-01
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Takayama, S;Yoshida, J;Murakami, S
  • 通讯作者:
    Murakami, S
先端歯科医学の創生(松岡 博)
创造先进的牙科(松冈浩)
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Tan;J.K.;Yamaguchi;I.;Ishikawa;S.;Naito;T.;Yokota;M.;Matsuo Yamamoto;村上 伸也
  • 通讯作者:
    村上 伸也
Origination of Frontier Bio Dentistry (Hiroshi Matsuoka)
Frontier Bio Dentistry 的起源(Hiroshi Matsuoka)
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Matin K;Senpuku H;Hanada N;Ozawa H;Ejiri S.;Shinya Murakami et al.
  • 通讯作者:
    Shinya Murakami et al.
再生医療工学の最先端(島 健太郎 )
再生医学工程的前沿(岛健太郎)
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    山口 生;タン ジュークイ;石川 聖二;内藤 徹;横田 誠;村上 伸也
  • 通讯作者:
    村上 伸也
Periodontal tissue regeneration by bFGF
bFGF 的牙周组织再生
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MURAKAMI Shinya其他文献

MURAKAMI Shinya的其他文献

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{{ truncateString('MURAKAMI Shinya', 18)}}的其他基金

Raman imaging of osteoblastic mineralization
成骨细胞矿化的拉曼成像
  • 批准号:
    25670883
  • 财政年份:
    2013
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Complexity single cell analysis designed to create a new periodontal regenerative medicine in silico
复杂的单细胞分析旨在通过计算机创建新的牙周再生医学
  • 批准号:
    23659917
  • 财政年份:
    2011
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of predictive periodontology based on molecular epidemiological study
基于分子流行病学研究的预测性牙周病学的发展
  • 批准号:
    23249086
  • 财政年份:
    2011
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular basis of predictable periodontal regeneration
可预测牙周再生的分子基础
  • 批准号:
    20390530
  • 财政年份:
    2008
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
CONSTRUCTION AND APPLICATION OF CDNA MICROARRAY SPECIALIZED FOR PERIODONTAL IIGAMENT
牙周韧带专用CDNA微阵列的构建及应用
  • 批准号:
    13557190
  • 财政年份:
    2001
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functions and regulatory mechanisms of adenosin in inflammatory periodontal tissues
腺苷在炎症性牙周组织中的功能及调控机制
  • 批准号:
    11470461
  • 财政年份:
    1999
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Establishment of periodontal regenerative therapy by basic libroblast growth lactor (bFGF)
碱性成纤维细胞生长因子(bFGF)牙周再生治疗的建立
  • 批准号:
    10557201
  • 财政年份:
    1998
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Characteristic Growth Factor Actions Involved in Periodontal Regeneration
生长因子参与牙周再生的特征性作用
  • 批准号:
    09671950
  • 财政年份:
    1997
  • 资助金额:
    $ 8.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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碱性成纤维细胞生长因子和牙釉质基质衍生物对大鼠皮下移植磨牙牙周组织再生的影响
  • 批准号:
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小热激蛋白在牙周组织再生中的表达机制分析
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基于amelogenin-CRP78复合物对牙周组织再生和难治性免疫疾病的挑战
  • 批准号:
    23H03084
  • 财政年份:
    2023
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Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication Approach
牙周组织再生的个性化策略——融合生物制造方法
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  • 财政年份:
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使用 LIPUS 和 BMP9 开发糖尿病患者牙周组织再生疗法
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  • 财政年份:
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用于牙周组织再生的新型骨替代物的制造
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