Molecular Design of Peptide-Material for Tissue Engineering

合成了与细胞粘附和铺展活性相关的多肽，并对其分子构象和细胞粘附和铺展活性进行了分析。对于Arg-Gly-Asp-Ser模拟肽，我们发现新设计的序列比天然序列具有更高的细胞附着活性，然后将它们杂交到高分子材料如聚乙烯醇的表面。对于层粘连蛋白相关肽，设计的肽表现出细胞附着和铺展活性。周期性多肽具有重复的氨基酸序列，这是发现在许多天然的周期性蛋白质，如胶原蛋白，合成和它们在溶液中的构象进行了分析的CD测量。结果表明，它们具有不同于已知的α-螺旋、β-链和聚脯氨酸-II结构的新的二级结构。

Peptides related to the cell-attachment and spreading activity were synthesized, and their molecular conformations and cell-attachment and spreading activity were analyzed. For Arg-Gly-Asp-Ser mimetic peptides, we found new designed sequences having higher cell-attachment activity than the native sequence, then they were hybridized on the surface of polymer materials such as poly(vinyl alcohol). For laminin related peptides, designed peptides exhibited the cell-attachment and spreading activity. Periodic polypeptides having repetitive amino-acid sequences, which are found in many native periodic proteins such as collagen, were synthesized and their conformations in solutions were analyzed by the CD measurement. It was shown that they take new secondary structures different from well-known α-helix, β-strand and polyproline-II structures.