Development of genetic and microbiological quality control system of mouse embryonic stem cell lines.

【导言】我们报道了在ICLAS监测中心发现的染色体（chr）异常和嵌合小鼠胚胎干细胞系的种系传播（GT）。【材料与方法】从18家机构共获得小鼠胚胎干细胞367株。采用Giemsa染色和QH染色进行核型分析，并检测嵌合小鼠ES细胞系的GT。[结果](1)67.0%（13所院校，246条线）的医院chr正常；15.5%（8所，57行）、9.0%（6所，33行）、3.8%（4所，14行）分别为41、42、39条。年代,分别。(2) 36株ES细胞株中4株携带40chr。S显示核型异常。1 / 4的核型为[40，X, +mar]。5个携带41chr的ES细胞系的核型。包括[41，XY, +8（3行）],[41，XY, +18（1行）]和[41，XY, +11（1行）]。携带42 chr的ES细胞系的核型。s包括[42，XY, +8, +11（2行）]和[42，XY, +6, +8（1行）]。(3) 2株ES细胞株均可见GT，分别为正常chr的80%和88%。数字。[讨论](1)对应。胚胎干细胞系中经常发生数量异常（33%）。(2)携带异常核型的细胞中，携带8和11三体的ES细胞占主导地位。(3)提示即使携带40 chrs的ES细胞系也应进行核型分析。

[Introduction] We report chromosomal(chr.) abnormalities found in the ICLAS Monitoring Center and germline transmission(GT) by ES cell lines in chimeric mice. [Materials and methods] A total of 367 mouse ES cell lines were obtained from 18 institutions. Karyotype analyses were performed by Giemsa staining and QH staining, and GT by the ES cell lines in chimeric mice was examined. [Results](1)Among the institusions, 67.0%(13 institutions, 246 lines) showed normal chr. numbers, and 15.5% (8 institutions, 57 lines), 9.0% (6 institutions, 33 lines) and 3.8%(4 institutions, 14 lines) showed 41, 42, and 39 chr.s, respectively. (2)4 of 36 ES cell lines carrying 40 chr.s showed karyotyping abnormalities. The karyotype of 1 out of 4 was [40, X, +mar]. The karyotypes of 5 ES cell lines carrying 41 chr.s included [41, XY, +8(3 lines)],[41, XY, +18(1 line)] and [41, XY, +11(1 line)]. The karyotypes of ES cell lines carrying 42 chr.s included [42, XY, +8, +11(2 lines)] and [42, XY, +6, +8(1 line)]. (3)GT was observed in 2 ES cell lines exhibiting 80 and 88% of normal chr. numbers. [Discussion](1)Chr. number abnormalities occur frequently in ES cell lines (33%). (2)Among the cells carrying abnormal karyotypes, ES cells carrying trisomy 8 and 11 were dominant. (3)It was suggested that karyotyping should be performed even in ES cell lines carrying 40 chr.s.